Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607031311_lijw ATATATCCAGTCAAGGAGCAATCACACTTTGG(+ATATAT)GATCCGCATCTCAAAGATAGGGTGAGAGAGG NGG 10 8 17 8 24 0 100 5 10
    240607031310_5tbb CATCTCCTCTCTCACCCTATCTTTGAGATGCG(+ATATAT)GATCCCAAAGTGTGATTGCTCCTTGACTGGA NGG 10 8 17 8 24 0 100 5 10
    240607031308_l2a2 AAAGGAAAGGTGTCTCTCCTACGGGCCAGGGG(+ATATAT)GATCCTATCACAAAAGAATAAAGCAGCCTGA NGG 10 8 17 8 24 0 100 5 10
    240607031307_ercf TCCAATCAGGCTGCTTTATTCTTTTGTGATAG(+ATATAT)GATCCCCCTGGCCCGTAGGAGAGACACCTTT NGG 10 8 17 8 24 0 100 5 10
    240607031305_uaoe AGGATCCCAGATCTCTTGGCCCATCCCATGAG(+ATATAT)GTACCTGACTGTCTACCAGCACTGTCTCTGG NGG 10 8 17 8 24 0 100 5 10
    240607031304_1otc GGCTACCAGAGACAGTGCTGGTAGACAGTCAG(+ATATAT)GTACCTCATGGGATGGGCCAAGAGATCTGGG NGG 10 8 17 8 24 0 100 5 10
    240607031303_glev CCCTGAGTGGGGTGGGTGGGGCTAGGAAGGAG(+ATATAT)GTACCCCACAGCTTTCTGCATCTATATGGAG NGG 10 8 17 8 24 0 100 5 10
    240607031301_oadm CTGGCCTCCATATAGATGCAGAAAGCTGTGGG(+ATATAT)GTACCTCCTTCCTAGCCCCACCCACCCCACT NGG 10 8 17 8 24 0 100 5 10
    240607031259_lcnw GTGTCAAATCCTCTTCTCTACATAGTTCCCAG(+ATATAT)GATCCCAGATCTCTTGGCCCATCCCATGAGG NGG 10 8 17 8 24 0 100 5 10
    240607031257_fobs AGGTACCTCATGGGATGGGCCAAGAGATCTGG(+ATATAT)GATCCTGGGAACTATGTAGAGAAGAGGATTT NGG 10 8 17 8 24 0 100 5 10