Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607031256_q5hu ATACTTAGGGTCACCAGGGGGAGCACTCTCCG(+ATATAT)GTACCCTTCACCTGGGTTTTAAATGGCTTCT NGG 10 8 17 8 24 0 100 5 10
    240607031254_fb76 CAGAGAGAAGCCATTTAAAACCCAGGTGAAGG(+ATATAT)GTACCGGAGAGTGCTCCCCCTGGTGACCCTA NGG 10 8 17 8 24 0 100 5 10
    240607031252_fwrm TTAAATGGCTTCTCTCTGTCTGGATCACCTAG(+ATATAT)GATCCAGTTTTAGTGAACCCTCTTCCAAAGA NGG 10 8 17 8 24 0 100 5 10
    240607031251_vl39 GTATCTCTTTGGAAGAGGGTTCACTAAAACTG(+ATATAT)GATCCTAGGTGATCCAGACAGAGAGAAGCCA NGG 10 8 17 8 24 0 100 5 10
    240607031249_f75w CAGCCCTAGGACCAAGAACAACATTGCCTTGG(+ATATAT)GTACCTTTGTAGTGAGTCACGGCTCTGGAGT NGG 10 8 17 8 24 0 100 5 10
    240607031248_uvat GCTGTACTCCAGAGCCGTGACTCACTACAAAG(+ATATAT)GTACCCAAGGCAATGTTGTTCTTGGTCCTAG NGG 10 8 17 8 24 0 100 5 10
    240607031246_e2sk TCCATCTCTAAGCATCGAAGCAAGGAGCCCTG(+ATATAT)GATCCCCAAGAGGTTCCTGAGTGCAGTTCCA NGG 10 8 17 8 24 0 100 5 10
    240607031244_lz10 AGTGCTGGAACTGCACTCAGGAACCTCTTGGG(+ATATAT)GATCCAGGGCTCCTTGCTTCGATGCTTAGAG NGG 10 8 17 8 24 0 100 5 10
    240607031243_gwle CGATGGAGCCTGATGACATTAGAGCCCTTTTG(+ATATAT)GTACCAGAAGGCAGCTGGAGCAGGGCCTGGG NGG 10 8 17 8 24 0 100 5 10
    240607031241_00ju TGGCTCCCAGGCCCTGCTCCAGCTGCCTTCTG(+ATATAT)GTACCAAAAGGGCTCTAATGTCATCAGGCTC NGG 10 8 17 8 24 0 100 5 10