Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607031223_hyuh GGGGACATTGTTAAATAGGGAAGGTGATGGTG(+ATATAT)GATCCTGACTGCTCCTACCCTGTGACCTTTA NGG 10 8 17 8 24 0 100 5 10
    240607031222_su3d ATGACTAAAGGTCACAGGGTAGGAGCAGTCAG(+ATATAT)GATCCACCATCACCTTCCCTATTTAACAATG NGG 10 8 17 8 24 0 100 5 10
    240607031220_z4sa ACAAGCACCCCTAAACCACCTCTGCCTACAGG(+ATATAT)GATCCCCAGGACCCCAATGGGCATCAGCCTT NGG 10 8 17 8 24 0 100 5 10
    240607031218_oatx GTTCAAAGGCTGATGCCCATTGGGGTCCTGGG(+ATATAT)GATCCCTGTAGGCAGAGGTGGTTTAGGGGTG NGG 10 8 17 8 24 0 100 5 10
    240607031217_qtpi CCTCAGGGTCAATGTTATACTCGGGTGTTGTG(+ATATAT)GATCCACCTGGGCCAGTGCATCTACATCTTC NGG 10 8 17 8 24 0 100 5 10
    240607031215_iykg CAGAAGAAGATGTAGATGCACTGGCCCAGGTG(+ATATAT)GATCCACAACACCCGAGTATAACATTGACCC NGG 10 8 17 8 24 0 100 5 10
    240607031214_i3pk GATTGTGGTTCAGTTTGTCTAATGTCAACTTG(+ATATAT)GTACCTACAGTAAAACTGTAAGTCCACTCGA NGG 10 8 17 8 24 0 100 5 10
    240607031213_27cv ATTTGTCGAGTGGACTTACAGTTTTACTGTAG(+ATATAT)GTACCAAGTTGACATTAGACAAACTGAACCA NGG 10 8 17 8 24 0 100 5 10
    240607031212_e46u CAGGTTTCATTTTAAGAAAGTCTTCCATGCTG(+ATATAT)GTACCCTGTTTTGTTCAATTTTTTTCTGCAT NGG 10 8 17 8 24 0 100 5 10
    240607031210_81eb AGTTTATGCAGAAAAAAATTGAACAAAACAGG(+ATATAT)GTACCAGCATGGAAGACTTTCTTAAAATGAA NGG 10 8 17 8 24 0 100 5 10