Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607031934_p106 TTGAGCGAGCGAGAGATTATTCTAATGTCCTG(+ATATAT)GTACCCGGCTGGCTTCTGGGAGGACTGGCTT NGG 10 8 17 8 24 0 100 5 10
    240607031932_ii7z TCGAAAAGCCAGTCCTCCCAGAAGCCAGCCGG(+ATATAT)GTACCAGGACATTAGAATAATCTCTCGCTCG NGG 10 8 17 8 24 0 100 5 10
    240607031931_6rp0 AAGCTTATTGGCCAAACCCTCAGGGCCTCTAG(+ATATAT)GTACCTCATTAGCATGGAGAACTCTGTTCTG NGG 10 8 17 8 24 0 100 5 10
    240607031930_ac4v ATCATCAGAACAGAGTTCTCCATGCTAATGAG(+ATATAT)GTACCTAGAGGCCCTGAGGGTTTGGCCAATA NGG 10 8 17 8 24 0 100 5 10
    240607031928_nhd4 TATGTGGGTGTGAGGTGGTCAGGCCATTTGTG(+ATATAT)GTACCCGTAGTGTTTGGTGCTGGCCCTAGCC NGG 10 8 17 8 24 0 100 5 10
    240607031927_xbd4 ATAGTGGCTAGGGCCAGCACCAAACACTACGG(+ATATAT)GTACCACAAATGGCCTGACCACCTCACACCC NGG 10 8 17 8 24 0 100 5 10
    240607031926_dpcg TGGAAGCAAGGATGAACGCTTTCCTAGGTCTG(+ATATAT)GTACCGTTTCCTCTCAATGGCCTCACCATAC NGG 10 8 17 8 24 0 100 5 10
    240607031925_xcmj ACTGAGTATGGTGAGGCCATTGAGAGGAAACG(+ATATAT)GTACCAGACCTAGGAAAGCGTTCATCCTTGC NGG 10 8 17 8 24 0 100 5 10
    240607031923_oml8 CACCCCTGGATGCTGTCTCCCAGCCAAAACTG(+ATATAT)GTACCTGTACTTACAGAAATCAAATTGCAGG NGG 10 8 17 8 24 0 100 5 10
    240607031922_it9o AATTGCCTGCAATTTGATTTCTGTAAGTACAG(+ATATAT)GTACCAGTTTTGGCTGGGAGACAGCATCCAG NGG 10 8 17 8 24 0 100 5 10