Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607032004_hkdn TGTTTTAGGCTGGTGGGAATAAAGATGCTATG(+ATATAT)GATCCTTTCTTTTCTTTTTTTTTTTAAGTAC NGG 10 8 17 8 24 0 100 5 10
    240607032003_14zi CAATAGTACTTAAAAAAAAAAAGAAAAGAAAG(+ATATAT)GATCCATAGCATCTTTATTCCCACCAGCCTA NGG 10 8 17 8 24 0 100 5 10
    240607032001_677i TGGTGGGCTCGGGTTTGCTTGGTCTTGTGAGG(+ATATAT)GTACCCATTGCCACTTTGAGTTTATGGGTTT NGG 10 8 17 8 24 0 100 5 10
    240607031959_37ih TAGCTAAACCCATAAACTCAAAGTGGCAATGG(+ATATAT)GTACCCTCACAAGACCAAGCAAACCCGAGCC NGG 10 8 17 8 24 0 100 5 10
    240607031958_iuk7 TGGTGGGCTCGGGCTTGCTTGGTCTTGTGCGG(+ATATAT)GTACCCATTGCCACTTTGAGTTTATGGGTTT NGG 10 8 17 8 24 0 100 5 10
    240607031956_epk2 TAGCTAAACCCATAAACTCAAAGTGGCAATGG(+ATATAT)GTACCCGCACAAGACCAAGCAAGCCCGAGCC NGG 10 8 17 8 24 0 100 5 10
    240607031955_wxqa TGGTTGGCTTGGGTTTGCTTGGTCTTGTGCGG(+ATATAT)GTACCCATTGCCACTTTGAGTTTATGGGTTT NGG 10 8 17 8 24 0 100 5 10
    240607031953_xr9r TAGCTAAACCCATAAACTCAAAGTGGCAATGG(+ATATAT)GTACCCGCACAAGACCAAGCAAACCCAAGCC NGG 10 8 17 8 24 0 100 5 10
    240607031952_8jfy CTTTCTTTCTTTTTTTTTTCTTTTTAAAATGG(+ATATAT)GATCCACTGTGTTGAAGACTCTTGATATCAT NGG 10 8 17 8 24 0 100 5 10
    240607031951_p4er AGCACATGATATCAAGAGTCTTCAACACAGTG(+ATATAT)GATCCCATTTTAAAAAGAAAAAAAAAAGAAA NGG 10 8 17 8 24 0 100 5 10