Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607032048_q969 CTTGTAGACATTTATCTTCAGGCGTCATCTTG(+ATATAT)GTACCAGTGGATCCCAGAGGACACACCCAGG NGG 10 8 17 8 24 0 100 5 10
    240607032047_60lz TTGGTCCTGGGTGTGTCCTCTGGGATCCACTG(+ATATAT)GTACCAAGATGACGCCTGAAGATAAATGTCT NGG 10 8 17 8 24 0 100 5 10
    240607032045_4a6l GTACATGGTGTTGAGGCACTGAGAGAACCAAG(+ATATAT)GATCCACTCTCAGTTCCTACCCAGAAGGGTC NGG 10 8 17 8 24 0 100 5 10
    240607032044_l3a8 TTCTGGACCCTTCTGGGTAGGAACTGAGAGTG(+ATATAT)GATCCTTGGTTCTCTCAGTGCCTCAACACCA NGG 10 8 17 8 24 0 100 5 10
    240607032042_4ovw GGCTTGCTGATTTCTAGTGTTCATCCCATTTG(+ATATAT)GATCCTTAAAGGTACCCCATCCCAGGAAGAA NGG 10 8 17 8 24 0 100 5 10
    240607032041_tnnu GCACCTTCTTCCTGGGATGGGGTACCTTTAAG(+ATATAT)GATCCAAATGGGATGAACACTAGAAATCAGC NGG 10 8 17 8 24 0 100 5 10
    240607032040_ir3h GCCTTAGTCCTTCCCTCTTTAATCCCCTATGG(+ATATAT)GATCCTTCTCTGTGTTCCTGTGTGTTTGCTT NGG 10 8 17 8 24 0 100 5 10
    240607032039_3gjd ATGAGAAGCAAACACACAGGAACACAGAGAAG(+ATATAT)GATCCCATAGGGGATTAAAGAGGGAAGGACT NGG 10 8 17 8 24 0 100 5 10
    240607032038_cg0m ACACTGAGGCTTCATTCTGTTCTTTAGCTTTG(+ATATAT)GTACCTTTAATCCAGAGTCAATGGGAAAAGA NGG 10 8 17 8 24 0 100 5 10
    240607032037_ysus CTATATCTTTTCCCATTGACTCTGGATTAAAG(+ATATAT)GTACCAAAGCTAAAGAACAGAATGAAGCCTC NGG 10 8 17 8 24 0 100 5 10