Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613184024_ar3w ATATGTGTACATCTGTATATATATTTGGTGGG(+AGGACA)GATCCTAAAGTCCGTGCTTTGACAGGCTTAT NGG 10 8 17 8 24 0 100 5 10
    240613184022_d7c8 ACTGTAAAGAAAGCTTCTAGGTCTTCAAAAGG(+AGGACA)GATCCAGAAAAGGGCAAGCCTGAAATGAGGA NGG 10 8 17 8 24 0 100 5 10
    240613184021_ugpt CTAGCTCCTCATTTCAGGCTTGCCCTTTTCTG(+AGGACA)GATCCCTTTTGAAGACCTAGAAGCTTTCTTT NGG 10 8 17 8 24 0 100 5 10
    240613184019_eshl ATGGAGAGAGGGAGACTAATTGGTAAGTGGAG(+AGGACA)GTACCTACCCAAGAAGTTCGGGTGACTCTTT NGG 10 8 17 8 24 0 100 5 10
    240613184018_d8bf TCTACAAAGAGTCACCCGAACTTCTTGGGTAG(+AGGACA)GTACCTCCACTTACCAATTAGTCTCCCTCTC NGG 10 8 17 8 24 0 100 5 10
    240613184016_ndbi GGGGTACATAATAAATAGTTCTCCATGAGGAG(+AGGACA)GATCCTAGGGTTATAAATAGGCAGTTCAACA NGG 10 8 17 8 24 0 100 5 10
    240613184013_ccth CCCTGTGTTGAACTGCCTATTTATAACCCTAG(+AGGACA)GATCCTCCTCATGGAGAACTATTTATTATGT NGG 10 8 17 8 24 0 100 5 10
    240613184012_cm0d AGAAAGAGACGGTGATGCCTCTAGGAATTCTG(+AGGACA)GATCCACGGGAGAAAAAGCAGTATCAGGCTG NGG 10 8 17 8 24 0 100 5 10
    240613184009_o3b8 GCGCGCAGCCTGATACTGCTTTTTCTCCCGTG(+AGGACA)GATCCAGAATTCCTAGAGGCATCACCGTCTC NGG 10 8 17 8 24 0 100 5 10
    240613184007_w2v8 TGCACAAGAGATGAGGAATATCAGAGAGATGG(+AGGACA)GATCCAATTCGATATTTAAACCTTTATCTGG NGG 10 8 17 8 24 0 100 5 10