Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613184058_w71d TTCCATTTCCCATCTCCTCAGGCTTGGGTCAG(+AGGACA)GATCCCCTGAATAGTCTTGGCTCTGTCTGTC NGG 10 8 17 8 24 0 100 5 10
    240613184057_kv00 ACATGAGCAAAGAACCTATTTCTTTCGTATGG(+AGGACA)GTACCTGAAATGTGTTCTCATTCCTGTGATA NGG 10 8 17 8 24 0 100 5 10
    240613184055_8cq2 GTGGGTATCACAGGAATGAGAACACATTTCAG(+AGGACA)GTACCCATACGAAAGAAATAGGTTCTTTGCT NGG 10 8 17 8 24 0 100 5 10
    240613184054_ecod TGCAAGAAGAACACTGCATACTGGGCATGCAG(+AGGACA)GTACCACCACCTGTCAGCAGTTTCTCCAGGC NGG 10 8 17 8 24 0 100 5 10
    240613184052_zgdf GCAGAGCCTGGAGAAACTGCTGACAGGTGGTG(+AGGACA)GTACCTGCATGCCCAGTATGCAGTGTTCTTC NGG 10 8 17 8 24 0 100 5 10
    240613184051_4ajn ACCCAGCTAGTGAGCTGTGCTCTGCAGGAGTG(+AGGACA)GATCCGAGTCTCCTAGATTGAGATTTGGAGG NGG 10 8 17 8 24 0 100 5 10
    240613184049_kyk0 GTGCACCTCCAAATCTCAATCTAGGAGACTCG(+AGGACA)GATCCACTCCTGCAGAGCACAGCTCACTAGC NGG 10 8 17 8 24 0 100 5 10
    240613184047_27jo AACTGTTCCCAAACATCAAGAATCTTTCCAAG(+AGGACA)GATCCTAAGGTGAAGCACTACCTACGATTTT NGG 10 8 17 8 24 0 100 5 10
    240613184045_tj15 AAGTGAAAATCGTAGGTAGTGCTTCACCTTAG(+AGGACA)GATCCTTGGAAAGATTCTTGATGTTTGGGAA NGG 10 8 17 8 24 0 100 5 10
    240613184043_0dna GTAGAGTTTTTATAAGGCCAGTGAGTTAACTG(+AGGACA)GTACCCTGTACTGCCAAATCTAATTTTTAGG NGG 10 8 17 8 24 0 100 5 10