Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613184115_sj5v GTCAACGAGCTCCCTGACCCATCACATCGTTG(+AGGACA)GATCCTCACAGGAGCCCTGTGAGCCAGGGAA NGG 10 8 17 8 24 0 100 5 10
    240613184114_dp3d TCAAGACATGAGTATGAGCACAGTTTCAAATG(+AGGACA)GTACCAAAATTAATCCATTTCACAGTAATCA NGG 10 8 17 8 24 0 100 5 10
    240613184112_45gz AGAAATGATTACTGTGAAATGGATTAATTTTG(+AGGACA)GTACCATTTGAAACTGTGCTCATACTCATGT NGG 10 8 17 8 24 0 100 5 10
    240613184111_w34x TGCTAAACTCCAAGCTGAAGGCACAATGAAGG(+AGGACA)GATCCTTGGTACCTTGAGCATTTCCTTTGAA NGG 10 8 17 8 24 0 100 5 10
    240613184109_4qz0 CCTTTTTCAAAGGAAATGCTCAAGGTACCAAG(+AGGACA)GATCCCTTCATTGTGCCTTCAGCTTGGAGTT NGG 10 8 17 8 24 0 100 5 10
    240613184107_fg7f TACCAAAAGGTCTGTCTGACCAGAGACTGAAG(+AGGACA)GTACCAAGGGAACTGGGCTTTTTTTCAGGCG NGG 10 8 17 8 24 0 100 5 10
    240613184105_hws8 CCCTGCGCCTGAAAAAAAGCCCAGTTCCCTTG(+AGGACA)GTACCTTCAGTCTCTGGTCAGACAGACCTTT NGG 10 8 17 8 24 0 100 5 10
    240613184103_4hhl TGGCCTTTGGTCCCAGACGGTCCAGGTCTGAG(+AGGACA)GATCCAATAGGGAAGCTGGAGCACTGGGAAA NGG 10 8 17 8 24 0 100 5 10
    240613184102_mazx GTCATTTTCCCAGTGCTCCAGCTTCCCTATTG(+AGGACA)GATCCTCAGACCTGGACCGTCTGGGACCAAA NGG 10 8 17 8 24 0 100 5 10
    240613184100_xkjy TTTGGGACAGACAGAGCCAAGACTATTCAGGG(+AGGACA)GATCCTGACCCAAGCCTGAGGAGATGGGAAA NGG 10 8 17 8 24 0 100 5 10