Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613184149_me9m TCTAGGGTTGGCCCTGGGAGTACCAAGAAAGG(+AGGACA)GTACCTTGGTTATCCTTGGTCCTCCCTAGGT NGG 10 8 17 8 24 0 100 5 10
    240613184148_6u1d CTTAGTCAGGGTCATAAGGTACTTTGGTGGTG(+AGGACA)GATCCTGCTCACTGTTATATAAGTTGGCAGG NGG 10 8 17 8 24 0 100 5 10
    240613184146_xi8f TGAGACCTGCCAACTTATATAACAGTGAGCAG(+AGGACA)GATCCACCACCAAAGTACCTTATGACCCTGA NGG 10 8 17 8 24 0 100 5 10
    240613184144_ctxe TCCAGAGGTTTTTACTGAGAGGACTGAGGGGG(+AGGACA)GTACCCCAGCTGGAGGGCTGAAGGCCCAGGA NGG 10 8 17 8 24 0 100 5 10
    240613184142_mdim GCCTTTCCTGGGCCTTCAGCCCTCCAGCTGGG(+AGGACA)GTACCCCCCTCAGTCCTCTCAGTAAAAACCT NGG 10 8 17 8 24 0 100 5 10
    240613184139_h4pv CCTTTTAGCTTGGAGGTGGGAGCCATGCCCAG(+AGGACA)GATCCCTTTCCCGGGCTTTGTCCATGGGAAA NGG 10 8 17 8 24 0 100 5 10
    240613184137_lr6j AGGGATTTCCCATGGACAAAGCCCGGGAAAGG(+AGGACA)GATCCTGGGCATGGCTCCCACCTCCAAGCTA NGG 10 8 17 8 24 0 100 5 10
    240613184135_0r24 TCTTTCCTCCAGCCCTTTTGGTCTCTTTCCTG(+AGGACA)GATCCTTGTCCTCCAGACTGGCCTCTGCTGG NGG 10 8 17 8 24 0 100 5 10
    240613184134_7uv7 ACTTTCCAGCAGAGGCCAGTCTGGAGGACAAG(+AGGACA)GATCCAGGAAAGAGACCAAAAGGGCTGGAGG NGG 10 8 17 8 24 0 100 5 10
    240613184132_9ojl GCCTTCCTCAGCCCTGACCTACCTGGCCATAG(+AGGACA)GATCCTACAGCCTTCTCTGTACTCCACTATC NGG 10 8 17 8 24 0 100 5 10