Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613184256_sw1o TAGTGAGACTCTGTCTCAAAAAAAAGGGGGGG(+AGGACA)GTACCAATGAAGAAGCATTAATGCATTATTT NGG 10 8 17 8 24 0 100 5 10
    240613184254_cm53 GGAATCTAGATTCCCCCAAGAGTTTAAGGAGG(+AGGACA)GATCCAGAAATCCAAATTTTAAGAACTACAG NGG 10 8 17 8 24 0 100 5 10
    240613184253_369d CAATCCTGTAGTTCTTAAAATTTGGATTTCTG(+AGGACA)GATCCCTCCTTAAACTCTTGGGGGAATCTAG NGG 10 8 17 8 24 0 100 5 10
    240613184252_9xe1 AAGGCTTCCTTCCATTAATAAATATCATGGTG(+AGGACA)GTACCCCCATGACTAAAAATTAGATTTTCAC NGG 10 8 17 8 24 0 100 5 10
    240613184249_4805 ATTCAGTGAAAATCTAATTTTTAGTCATGGGG(+AGGACA)GTACCACCATGATATTTATTAATGGAAGGAA NGG 10 8 17 8 24 0 100 5 10
    240613184247_2kwi GTCTTCTGGGAACAGCATTATACCATTGCAGG(+AGGACA)GATCCTCTGCTGAAACTTTTTTAATACTATA NGG 10 8 17 8 24 0 100 5 10
    240613184246_wkoi AAAAATATAGTATTAAAAAAGTTTCAGCAGAG(+AGGACA)GATCCCTGCAATGGTATAATGCTGTTCCCAG NGG 10 8 17 8 24 0 100 5 10
    240613184244_4yw4 ATGTACCCACATCCCCACAAGGGAACTTTAAG(+AGGACA)GATCCACCCCTTAAGCCCCTCCCTCCTCTGT NGG 10 8 17 8 24 0 100 5 10
    240613184242_wk24 TTACAACAGAGGAGGGAGGGGCTTAAGGGGTG(+AGGACA)GATCCTTAAAGTTCCCTTGTGGGGATGTGGG NGG 10 8 17 8 24 0 100 5 10
    240613184240_txbk TCAACCTGGTGGCTCACAACTATCTGTAATGG(+AGGACA)GATCCGATGCCCTTTTCTGATGTGTCTAAAG NGG 10 8 17 8 24 0 100 5 10