Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613184402_jlea GTAGGGTCTCTCCAGTTACTTACCCTGAACTG(+AGGACA)GTACCTAACCTGGAAAAAGGACTTTACTACT NGG 10 8 17 8 24 0 100 5 10
    240613184400_98qe GCTCACAAACATCTATAAATGTAGCTCCATAG(+AGGACA)GATCCAATGCCCCCATCTGCATGCAGACAAA NGG 10 8 17 8 24 0 100 5 10
    240613184358_ep3t GGCATTTTGTCTGCATGCAGATGGGGGCATTG(+AGGACA)GATCCTATGGAGCTACATTTATAGATGTTTG NGG 10 8 17 8 24 0 100 5 10
    240613184356_xg9r GAACACTTTTTGTTTTATTATTCTAGTCTGGG(+AGGACA)GATCCAGATTCCATAGCCATGGGACTGCTGG NGG 10 8 17 8 24 0 100 5 10
    240613184355_veqd CAGGCCCAGCAGTCCCATGGCTATGGAATCTG(+AGGACA)GATCCCCAGACTAGAATAATAAAACAAAAAG NGG 10 8 17 8 24 0 100 5 10
    240613184353_92nu TACAGCATCTGCCATGCTCCCTTCCATAGCCG(+AGGACA)GTACCTTAAGTGAGTTCAATAGTTCCTTTGA NGG 10 8 17 8 24 0 100 5 10
    240613184352_8hpe GCCTTTCAAAGGAACTATTGAACTCACTTAAG(+AGGACA)GTACCGGCTATGGAAGGGAGCATGGCAGATG NGG 10 8 17 8 24 0 100 5 10
    240613184351_tbhw CAAAAATGAAGAGAGAAAACAGGCCTATTGTG(+AGGACA)GTACCTTCTCAACAAGGCTTTCAGTAAGAGT NGG 10 8 17 8 24 0 100 5 10
    240613184349_9lm7 AGCAAACTCTTACTGAAAGCCTTGTTGAGAAG(+AGGACA)GTACCACAATAGGCCTGTTTTCTCTCTTCAT NGG 10 8 17 8 24 0 100 5 10
    240613184347_umzp GTAGCTCTTTTCTGTGAGACTCCCAACAGTGG(+AGGACA)GTACCGTGTTTGGCTGATATCCCTGGGAGGT NGG 10 8 17 8 24 0 100 5 10