Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613184411_v4v4 CAGTGTTCAAACCATACTAACACGACTTGATG(+AGGACA)GTACCCAAAAATATCAACCAACAAAATTTCA NGG 10 8 17 8 24 0 100 5 10
    240613184410_e3ci GAAAATGAAATTTTGTTGGTTGATATTTTTGG(+AGGACA)GTACCATCAAGTCGTGTTAGTATGGTTTGAA NGG 10 8 17 8 24 0 100 5 10
    240613184409_ccyk ATCTAGAGGAGGACCTCTAATTTACATACATG(+AGGACA)GATCCATCAACATTCATACATTTATTTACTT NGG 10 8 17 8 24 0 100 5 10
    240613184407_nabd GATGGAAGTAAATAAATGTATGAATGTTGATG(+AGGACA)GATCCATGTATGTAAATTAGAGGTCCTCCTC NGG 10 8 17 8 24 0 100 5 10
    240613184406_wkv0 AGATGGTCACCAACTCTGTAATCTGTAGAGTG(+AGGACA)GATCCACTTGCTCGAGTCACTGGGTCACACG NGG 10 8 17 8 24 0 100 5 10
    240613184405_sxlz CTATTCGTGTGACCCAGTGACTCGAGCAAGTG(+AGGACA)GATCCACTCTACAGATTACAGAGTTGGTGAC NGG 10 8 17 8 24 0 100 5 10
    240613184403_s2o9 TCTGAAGTAGTAAAGTCCTTTTTCCAGGTTAG(+AGGACA)GTACCAGTTCAGGGTAAGTAACTGGAGAGAC NGG 10 8 17 8 24 0 100 5 10
    240613184402_jlea GTAGGGTCTCTCCAGTTACTTACCCTGAACTG(+AGGACA)GTACCTAACCTGGAAAAAGGACTTTACTACT NGG 10 8 17 8 24 0 100 5 10
    240613184400_98qe GCTCACAAACATCTATAAATGTAGCTCCATAG(+AGGACA)GATCCAATGCCCCCATCTGCATGCAGACAAA NGG 10 8 17 8 24 0 100 5 10
    240613184358_ep3t GGCATTTTGTCTGCATGCAGATGGGGGCATTG(+AGGACA)GATCCTATGGAGCTACATTTATAGATGTTTG NGG 10 8 17 8 24 0 100 5 10