Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613184313_o188 GGGGGGGGTAGGGAGACAACTCATGAAGAAAG(+AGGACA)GTACCCTGTCTTTCCCCTTTGTGTTGGGGTC NGG 10 8 17 8 24 0 100 5 10
    240613184310_ziaq TGGAGAGGCCAGGACCCCACCTGCTGCATGGG(+AGGACA)GATCCACTAGCCATGGAAGGATACAATAGGA NGG 10 8 17 8 24 0 100 5 10
    240613184308_pcxo AGGCTTCCTATTGTATCCTTCCATGGCTAGTG(+AGGACA)GATCCCCATGCAGCAGGTGGGGTCCTGGCCT NGG 10 8 17 8 24 0 100 5 10
    240613184307_jnp9 TTCCATTCAGAAGAATGGGACACAGCTGACAG(+AGGACA)GATCCAGTGCTTGCTAGATTTCATCAGTTCT NGG 10 8 17 8 24 0 100 5 10
    240613184306_31pv TCTGAAGAACTGATGAAATCTAGCAAGCACTG(+AGGACA)GATCCTGTCAGCTGTGTCCCATTCTTCTGAA NGG 10 8 17 8 24 0 100 5 10
    240613184305_1gu5 TACATTATAAGCTATTTTCTGATTCTGTAATG(+AGGACA)GTACCCTTGCTCTCATTTTAAAGAAGTTTTT NGG 10 8 17 8 24 0 100 5 10
    240613184303_lxqh AGATTAAAAACTTCTTTAAAATGAGAGCAAGG(+AGGACA)GTACCATTACAGAATCAGAAAATAGCTTATA NGG 10 8 17 8 24 0 100 5 10
    240613184301_a7v6 GACAAATCTATCTGATGGGACAAGGAGCACCG(+AGGACA)GATCCTCTCTCGGGTTCTTCCTAGGACCAGC NGG 10 8 17 8 24 0 100 5 10
    240613184300_wu9x GCCAAGCTGGTCCTAGGAAGAACCCGAGAGAG(+AGGACA)GATCCGGTGCTCCTTGTCCCATCAGATAGAT NGG 10 8 17 8 24 0 100 5 10
    240613184259_sql5 AAAATAAATAATGCATTAATGCTTCTTCATTG(+AGGACA)GTACCCCCCCCTTTTTTTTGAGACAGAGTCT NGG 10 8 17 8 24 0 100 5 10