Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613184346_8u3f GGCAGACCTCCCAGGGATATCAGCCAAACACG(+AGGACA)GTACCCACTGTTGGGAGTCTCACAGAAAAGA NGG 10 8 17 8 24 0 100 5 10
    240613184344_ekk3 GCCACCATACCTAACTTTTACATGGGTGCTAG(+AGGACA)GATCCAAATTCTGGTCCTCCTGCTTCAACTG NGG 10 8 17 8 24 0 100 5 10
    240613184342_f1he TGGCCCAGTTGAAGCAGGAGGACCAGAATTTG(+AGGACA)GATCCTAGCACCCATGTAAAAGTTAGGTATG NGG 10 8 17 8 24 0 100 5 10
    240613184341_0pjn CAAAGGAGATGTAAAAGGGGTAATAGGAGGAG(+AGGACA)GATCCGAGGATTCTGCTGAAGGCCGGGGGTT NGG 10 8 17 8 24 0 100 5 10
    240613184338_silx AGTGCAACCCCCGGCCTTCAGCAGAATCCTCG(+AGGACA)GATCCTCCTCCTATTACCCCTTTTACATCTC NGG 10 8 17 8 24 0 100 5 10
    240613184336_mjro GCCCTAGGATTATTTGACTACTGGCCCCAAAG(+AGGACA)GTACCTAAAGGTCAAAATATTTTTCTATCGA NGG 10 8 17 8 24 0 100 5 10
    240613184335_cleo CTTTGTCGATAGAAAAATATTTTGACCTTTAG(+AGGACA)GTACCTTTGGGGCCAGTAGTCAAATAATCCT NGG 10 8 17 8 24 0 100 5 10
    240613184333_tskw ACTGCCCCTGGAATATAAAGCCTTTGATATGG(+AGGACA)GTACCCTGTCTCATGGCCTCTTCTTGGTCGT NGG 10 8 17 8 24 0 100 5 10
    240613184331_v0v0 TGGAAACGACCAAGAAGAGGCCATGAGACAGG(+AGGACA)GTACCCATATCAAAGGCTTTATATTCCAGGG NGG 10 8 17 8 24 0 100 5 10
    240613184330_70jp TAATGAGCCATGGACACTGACCCACCACTGTG(+AGGACA)GATCCACAGACTCAAACAAGGCCCTGATGGC NGG 10 8 17 8 24 0 100 5 10