Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607031109_9r8h TTTTACTCCCAAGACCTCAGGGGGAGTAACTG(+ATATAT)GTACCCAAAGTACCACTTGGTCTGAGCCTTC NGG 10 8 17 8 24 0 100 5 10
    240607031108_my5h AGAGAGAAGGCTCAGACCAAGTGGTACTTTGG(+ATATAT)GTACCAGTTACTCCCCCTGAGGTCTTGGGAG NGG 10 8 17 8 24 0 100 5 10
    240607031106_k4ho AGTCTTGGGAGAGGCTCAGGATGGAATTTTGG(+ATATAT)GATCCCTGGGGTCTTTGGTACGGAGGATAAG NGG 10 8 17 8 24 0 100 5 10
    240607031103_xp2v GTCACCTTATCCTCCGTACCAAAGACCCCAGG(+ATATAT)GATCCCAAAATTCCATCCTGAGCCTCTCCCA NGG 10 8 17 8 24 0 100 5 10
    240607031101_jpwb GGAAAGTGGGCCATAGAGGGGAAGGCCACAGG(+ATATAT)GATCCTGCTGAGAGCCCTGCCTTACATAATG NGG 10 8 17 8 24 0 100 5 10
    240607031100_n9nh GCAGACATTATGTAAGGCAGGGCTCTCAGCAG(+ATATAT)GATCCCTGTGGCCTTCCCCTCTATGGCCCAC NGG 10 8 17 8 24 0 100 5 10
    240607031059_qzmy TTCCATTCTCTATGTTGTTAGTAAATACTTTG(+ATATAT)GATCCCGGCTAGTGTTCTCTACCCTACTCCC NGG 10 8 17 8 24 0 100 5 10
    240607031057_x6se CAGAGGGGAGTAGGGTAGAGAACACTAGCCGG(+ATATAT)GATCCAAAGTATTTACTAACAACATAGAGAA NGG 10 8 17 8 24 0 100 5 10
    240607031056_9yuk TGTGGGAAGGGGTGTCGGCATACAGATCAGTG(+ATATAT)GTACCACCCACCTTCTGATGTAACATGTTCC NGG 10 8 17 8 24 0 100 5 10
    240607031054_fcui GAGTGGGAACATGTTACATCAGAAGGTGGGTG(+ATATAT)GTACCACTGATCTGTATGCCGACACCCCTTC NGG 10 8 17 8 24 0 100 5 10