Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607031022_0mih TCATTTTGGACATATCTTTGGAGAAATGATTG(+ATATAT)GATCCAACATTTTATAAACCAGGTAAAATTT NGG 10 8 17 8 24 0 100 5 10
    240607031021_wy3i ACCAGAAATTTTACCTGGTTTATAAAATGTTG(+ATATAT)GATCCAATCATTTCTCCAAAGATATGTCCAA NGG 10 8 17 8 24 0 100 5 10
    240607031020_20kv GATCTTGAGTTTTGCACCAAGAGGGAGGAGTG(+ATATAT)GATCCAAAAAAAAATACACAAACAAACAAAC NGG 10 8 17 8 24 0 100 5 10
    240607031019_w0ka TGTTTGTTTGTTTGTTTGTGTATTTTTTTTTG(+ATATAT)GATCCACTCCTCCCTCTTGGTGCAAAACTCA NGG 10 8 17 8 24 0 100 5 10
    240607031018_na9o CCTGTTCTGAATATTTCATACAGACGTGACTG(+ATATAT)GATCCTTTTAACTGGCCAGTGATTTCAAGGA NGG 10 8 17 8 24 0 100 5 10
    240607031016_1blx GAATATCCTTGAAATCACTGGCCAGTTAAAAG(+ATATAT)GATCCAGTCACGTCTGTATGAAATATTCAGA NGG 10 8 17 8 24 0 100 5 10
    240607031015_h8b6 ATTTCCTGCCCTTGGGACTATTTAGACAAGGG(+ATATAT)GATCCCAAAACTGGGCAGAACAAATGAACCA NGG 10 8 17 8 24 0 100 5 10
    240607031013_axxx CCTGGTGGTTCATTTGTTCTGCCCAGTTTTGG(+ATATAT)GATCCCCTTGTCTAAATAGTCCCAAGGGCAG NGG 10 8 17 8 24 0 100 5 10
    240607031012_4ng3 AGGCAGGGGAAGGATTTATCAAGAGATTCTTG(+ATATAT)GTACCAGCAGTAGCGACCACATGCACTGAGT NGG 10 8 17 8 24 0 100 5 10
    240607031011_syq3 GCTCAACTCAGTGCATGTGGTCGCTACTGCTG(+ATATAT)GTACCAAGAATCTCTTGATAAATCCTTCCCC NGG 10 8 17 8 24 0 100 5 10