Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607030923_kt84 TGTTACTACATCTGGCTGCAGTAAGACTTTTG(+ATATAT)GATCCAATGTAAACAGGAAGGGTAAGCATGT NGG 10 8 17 8 24 0 100 5 10
    240607030922_tewc GGGGAACATGCTTACCCTTCCTGTTTACATTG(+ATATAT)GATCCAAAAGTCTTACTGCAGCCAGATGTAG NGG 10 8 17 8 24 0 100 5 10
    240607030921_2q03 TGTATGTATGTGTGCATATCATGTGTGTTCTG(+ATATAT)GTACCTGTGGAGATTAGGAGAGACTGTCCGG NGG 10 8 17 8 24 0 100 5 10
    240607030919_81p1 GGGCACCGGACAGTCTCTCCTAATCTCCACAG(+ATATAT)GTACCAGAACACACATGATATGCACACATAC NGG 10 8 17 8 24 0 100 5 10
    240607030918_or8q AGAGGCCGGGTTTACTTTGATAGGCTAAATAG(+ATATAT)GTACCCCAGTTAGCTGTTTGTTTTGGATTTG NGG 10 8 17 8 24 0 100 5 10
    240607030916_qkef ATTCTCAAATCCAAAACAAACAGCTAACTGGG(+ATATAT)GTACCTATTTAGCCTATCAAAGTAAACCCGG NGG 10 8 17 8 24 0 100 5 10
    240607030915_0j0k GGCCAGACAAGGCGGCCCAGTTAAGAGAACAG(+ATATAT)GATCCACAGGCAGCTCCCTGCTCCAGTTGTT NGG 10 8 17 8 24 0 100 5 10
    240607030913_kr5o TCCTCAACAACTGGAGCAGGGAGCTGCCTGTG(+ATATAT)GATCCTGTTCTCTTAACTGGGCCGCCTTGTC NGG 10 8 17 8 24 0 100 5 10
    240607030912_h1ew TTGTTGAAGCTGCTGGCCACCAGCAATGATAG(+ATATAT)GATCCAATGGCGAGTTGGGCCGTATCTGAGA NGG 10 8 17 8 24 0 100 5 10
    240607030910_tro1 ATGGGTCTCAGATACGGCCCAACTCGCCATTG(+ATATAT)GATCCTATCATTGCTGGTGGCCAGCAGCTTC NGG 10 8 17 8 24 0 100 5 10