Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607031009_5369 GTGAAGGAATCACTCCTGGTGCAATACTGTGG(+ATATAT)GTACCAAGGCCTGTCTAAGCCACTTCAGGAG NGG 10 8 17 8 24 0 100 5 10
    240607031007_8jnq GATCCCTCCTGAAGTGGCTTAGACAGGCCTTG(+ATATAT)GTACCCACAGTATTGCACCAGGAGTGATTCC NGG 10 8 17 8 24 0 100 5 10
    240607031005_118q CACGCCGGCTCCTGTTTAGAAAGCGGGTGGGG(+ATATAT)GATCCACAGAACACACATGCAGAATGCATCC NGG 10 8 17 8 24 0 100 5 10
    240607031003_l6yg GATCAGGATGCATTCTGCATGTGTGTTCTGTG(+ATATAT)GATCCCCCACCCGCTTTCTAAACAGGAGCCG NGG 10 8 17 8 24 0 100 5 10
    240607031001_e6e1 ATTCCCTTCACAGTGCCATAAACCGACCCTGG(+ATATAT)GATCCCACATTCCCAGATAATTATAAGTCTG NGG 10 8 17 8 24 0 100 5 10
    240607030959_mae3 ACAAGCAGACTTATAATTATCTGGGAATGTGG(+ATATAT)GATCCCAGGGTCGGTTTATGGCACTGTGAAG NGG 10 8 17 8 24 0 100 5 10
    240607030958_kpmd AACCACATGGTGGTAACAACCATCTGTAATGG(+ATATAT)GATCCGTTGCCCTCTTCTGGCGTGTCTAAAG NGG 10 8 17 8 24 0 100 5 10
    240607030957_2wiq GCGGTCTTTAGACACGCCAGAAGAGGGCAACG(+ATATAT)GATCCCATTACAGATGGTTGTTACCACCATG NGG 10 8 17 8 24 0 100 5 10
    240607030956_qt95 AACCTGAATTTCATCCTCGGGACTCAATAGTG(+ATATAT)GATCCAGAGAACCAACTTCTAAAACTTGTCC NGG 10 8 17 8 24 0 100 5 10
    240607030954_ux89 AGTCAGGACAAGTTTTAGAAGTTGGTTCTCTG(+ATATAT)GATCCACTATTGAGTCCCGAGGATGAAATTC NGG 10 8 17 8 24 0 100 5 10