Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607030953_dwsd GTGTGTGCATTTACCCACAGAACAAGGCATTG(+ATATAT)GATCCCTTGGCACTGGTGTCTCTGTCTCTCT NGG 10 8 17 8 24 0 100 5 10
    240607030951_wm35 GAGACAGAGAGACAGAGACACCAGTGCCAAGG(+ATATAT)GATCCAATGCCTTGTTCTGTGGGTAAATGCA NGG 10 8 17 8 24 0 100 5 10
    240607030949_9tii TGTTCTCGGCATTAGTTTTCAAAATGTCCATG(+ATATAT)GATCCCTCGTCATCTGAGGAGCTGATTGTAG NGG 10 8 17 8 24 0 100 5 10
    240607030948_37is CCCTCCTACAATCAGCTCCTCAGATGACGAGG(+ATATAT)GATCCATGGACATTTTGAAAACTAATGCCGA NGG 10 8 17 8 24 0 100 5 10
    240607030946_20su TATGAAAATGGAGGGTAGACAACAGGTATCAG(+ATATAT)GTACCCACAAAATGAACCTCACCATGAAGTA NGG 10 8 17 8 24 0 100 5 10
    240607030945_vxhl TGTGGTACTTCATGGTGAGGTTCATTTTGTGG(+ATATAT)GTACCTGATACCTGTTGTCTACCCTCCATTT NGG 10 8 17 8 24 0 100 5 10
    240607030943_c6dj GTTCTGAAGTAGATGGTTGCCATGAACTCCCG(+ATATAT)GATCCAGATAGTCTCATTCATGATGGAGGTG NGG 10 8 17 8 24 0 100 5 10
    240607030941_367m GGGATCACCTCCATCATGAATGAGACTATCTG(+ATATAT)GATCCGGGAGTTCATGGCAACCATCTACTTC NGG 10 8 17 8 24 0 100 5 10
    240607030940_07o4 AATGAAAAGCAGCTAACTACAGAAGCAACATG(+ATATAT)GATCCTGCTCTGATTACCCATCGTCCCTAGA NGG 10 8 17 8 24 0 100 5 10
    240607030939_l7o3 TCAGCTCTAGGGACGATGGGTAATCAGAGCAG(+ATATAT)GATCCATGTTGCTTCTGTAGTTAGCTGCTTT NGG 10 8 17 8 24 0 100 5 10