Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607030746_0yco TTATTAAAATTTCACACAAACACAAGCACAGG(+ATATAT)GTACCAGAGCTCTCTTCCTGGATACCAGGTA NGG 10 8 17 8 24 0 100 5 10
    240607030745_dbkj AAAGCTACCTGGTATCCAGGAAGAGAGCTCTG(+ATATAT)GTACCCTGTGCTTGTGTTTGTGTGAAATTTT NGG 10 8 17 8 24 0 100 5 10
    240607030744_kckj CTTTTGATGTGGATAATGTGTCAAACTATGTG(+ATATAT)GATCCCAATGTCAGGATGAAAAAAAGTATCC NGG 10 8 17 8 24 0 100 5 10
    240607030742_oskj AGCATGGATACTTTTTTTCATCCTGACATTGG(+ATATAT)GATCCACATAGTTTGACACATTATCCACATC NGG 10 8 17 8 24 0 100 5 10
    240607030740_njag ATGAGATGAAATATTTGCTTCGCACACGCAGG(+ATATAT)GATCCCACTATAGAAGTTCTCTTCAAAGGAG NGG 10 8 17 8 24 0 100 5 10
    240607030739_t46q AAGGTCTCCTTTGAAGAGAACTTCTATAGTGG(+ATATAT)GATCCCTGCGTGTGCGAAGCAAATATTTCAT NGG 10 8 17 8 24 0 100 5 10
    240607030738_uzbx TGTTAGGAGTAGGTTCCTGGGCCTGGAAACAG(+ATATAT)GATCCTGGTTCAACCCTTCCAGCAAGATGGA NGG 10 8 17 8 24 0 100 5 10
    240607030736_5jao GCTTCTCCATCTTGCTGGAAGGGTTGAACCAG(+ATATAT)GATCCTGTTTCCAGGCCCAGGAACCTACTCC NGG 10 8 17 8 24 0 100 5 10
    240607030734_5yr3 CCTTGATGCAGTCTGTGCCTGACCGCTGCCTG(+ATATAT)GTACCAACAGTAACACTACAAAAGGCTCTCT NGG 10 8 17 8 24 0 100 5 10
    240607030733_nrzw AGTCCAGAGAGCCTTTTGTAGTGTTACTGTTG(+ATATAT)GTACCAGGCAGCGGTCAGGCACAGACTGCAT NGG 10 8 17 8 24 0 100 5 10