Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607030716_jiqc CCAAGTGCAGAGCCAGTGTTGGACCACCTCAG(+ATATAT)GATCCCCTTGTGGAGACTGCCTGGTGACCTC NGG 10 8 17 8 24 0 100 5 10
    240607030714_9pvj ATTCAGAGGTCACCAGGCAGTCTCCACAAGGG(+ATATAT)GATCCTGAGGTGGTCCAACACTGGCTCTGCA NGG 10 8 17 8 24 0 100 5 10
    240607030713_06id GCCGCAGGCGCCCAAGCGGACTCACCCGGCAG(+ATATAT)GATCCGAGTCCTAGGCGCGGCACAGTGGCGG NGG 10 8 17 8 24 0 100 5 10
    240607030711_az19 CACCGCCGCCACTGTGCCGCGCCTAGGACTCG(+ATATAT)GATCCTGCCGGGTGAGTCCGCTTGGGCGCCT NGG 10 8 17 8 24 0 100 5 10
    240607030710_g6n2 TAAAATAATGAGACAGTTTTTTGTTATTGGTG(+ATATAT)GATCCAGGAGCTGTTTAGAAACTATGTATTT NGG 10 8 17 8 24 0 100 5 10
    240607030708_joej GGGGAAAATACATAGTTTCTAAACAGCTCCTG(+ATATAT)GATCCACCAATAACAAAAAACTGTCTCATTA NGG 10 8 17 8 24 0 100 5 10
    240607030706_9phu TGGGGCCCAGCTGCGGCTGGCTCCCGGCCTGG(+ATATAT)GTACCAGAAAGAGACCTTCAGCTCAACCCAG NGG 10 8 17 8 24 0 100 5 10
    240607030704_kou6 CCAGCCTGGGTTGAGCTGAAGGTCTCTTTCTG(+ATATAT)GTACCCAGGCCGGGAGCCAGCCGCAGCTGGG NGG 10 8 17 8 24 0 100 5 10
    240607030703_cn4z TCTGTTGTTACTAATAACAGACTTTATCATGG(+ATATAT)GTACCAGCCATAGCGAGTTAATAAGTGACAT NGG 10 8 17 8 24 0 100 5 10
    240607030701_i8x3 TGGATATGTCACTTATTAACTCGCTATGGCTG(+ATATAT)GTACCCATGATAAAGTCTGTTATTAGTAACA NGG 10 8 17 8 24 0 100 5 10