Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607030700_pbjd TTCGACATTTTAACCTGTTCTGCCTGACATGG(+ATATAT)GATCCTCCATCCCAGCTCCTTCCCCAACACT NGG 10 8 17 8 24 0 100 5 10
    240607030658_dn94 AGCTCAGTGTTGGGGAAGGAGCTGGGATGGAG(+ATATAT)GATCCCATGTCAGGCAGAACAGGTTAAAATG NGG 10 8 17 8 24 0 100 5 10
    240607030656_vo5h TTGATGCCTTCTTTTAAAGTACCCTGCTCCTG(+ATATAT)GTACCCAGTTAATCCCCTCTCCCAGTATAAA NGG 10 8 17 8 24 0 100 5 10
    240607030655_86wf CCGACTTTATACTGGGAGAGGGGATTAACTGG(+ATATAT)GTACCAGGAGCAGGGTACTTTAAAAGAAGGC NGG 10 8 17 8 24 0 100 5 10
    240607030653_2x8p GTGCAACCTGTTACCTTACACAGTGCCTCATG(+ATATAT)GTACCCTGGGAGTGTGCGGGCATCATTCCCA NGG 10 8 17 8 24 0 100 5 10
    240607030651_7wug ACGGTTGGGAATGATGCCCGCACACTCCCAGG(+ATATAT)GTACCATGAGGCACTGTGTAAGGTAACAGGT NGG 10 8 17 8 24 0 100 5 10
    240607030650_vzb4 GTGAGTTTACCATTTCACATTATCATCACTAG(+ATATAT)GATCCTCTCCTTTCCAGATGGAAGCTCACAC NGG 10 8 17 8 24 0 100 5 10
    240607030648_h2p7 CATGGGTGTGAGCTTCCATCTGGAAAGGAGAG(+ATATAT)GATCCTAGTGATGATAATGTGAAATGGTAAA NGG 10 8 17 8 24 0 100 5 10
    240607030647_3oa5 AAAATTACATGTCCATGAACAGACTTTTCCTG(+ATATAT)GATCCAGACCACAAAAAGGGAATTATTTTTC NGG 10 8 17 8 24 0 100 5 10
    240607030645_o024 TATGTGAAAAATAATTCCCTTTTTGTGGTCTG(+ATATAT)GATCCAGGAAAAGTCTGTTCATGGACATGTA NGG 10 8 17 8 24 0 100 5 10