Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607030631_0547 TTCCAAGGCTAACATGAGAGATGTTACAGATG(+ATATAT)GTACCTCAAAGTTTTTCAAATTCACTTGACT NGG 10 8 17 8 24 0 100 5 10
    240607030630_k4e3 TGATCAGTCAAGTGAATTTGAAAAACTTTGAG(+ATATAT)GTACCATCTGTAACATCTCTCATGTTAGCCT NGG 10 8 17 8 24 0 100 5 10
    240607030628_m3so TTCCAAGGCTAACATGAGAGATGTTACAGATG(+ATATAT)GTACCTCAAAGTTTTTCAAATTCACTTGACT NGG 10 8 17 8 24 0 100 5 10
    240607030627_s6w8 TGATCAGTCAAGTGAATTTGAAAAACTTTGAG(+ATATAT)GTACCATCTGTAACATCTCTCATGTTAGCCT NGG 10 8 17 8 24 0 100 5 10
    240607030626_ujzt ATGTATGTTTTGCCTGCCACAACCCGTACCTG(+ATATAT)GTACCAGCAAAACCTGCAAAAAGCAAACAAG NGG 10 8 17 8 24 0 100 5 10
    240607030624_827l CCTGGCTTGTTTGCTTTTTGCAGGTTTTGCTG(+ATATAT)GTACCAGGTACGGGTTGTGGCAGGCAAAACA NGG 10 8 17 8 24 0 100 5 10
    240607030622_57jj AGACTCATTAATAGGATTGTTAGCAACCACTG(+ATATAT)GATCCTCTGGTGGATAGACAAATGTTCTATA NGG 10 8 17 8 24 0 100 5 10
    240607030621_yweh TTCCATATAGAACATTTGTCTATCCACCAGAG(+ATATAT)GATCCAGTGGTTGCTAACAATCCTATTAATG NGG 10 8 17 8 24 0 100 5 10
    240607030619_1dxe ATGAGATGAAATATTTGCTTCGCACACGCAGG(+ATATAT)GATCCCACTATAGAAGTTCTCTTCAAAGGAG NGG 10 8 17 8 24 0 100 5 10
    240607030617_q41c AAGGTCTCCTTTGAAGAGAACTTCTATAGTGG(+ATATAT)GATCCCTGCGTGTGCGAAGCAAATATTTCAT NGG 10 8 17 8 24 0 100 5 10