Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607030616_a7u0 AAGTGAAGGCTATGATTATTTCTCTAGTGCAG(+ATATAT)GATCCGGACAGGGTTCCACAAATCAATTCCT NGG 10 8 17 8 24 0 100 5 10
    240607030614_klxy GATATAGGAATTGATTTGTGGAACCCTGTCCG(+ATATAT)GATCCTGCACTAGAGAAATAATCATAGCCTT NGG 10 8 17 8 24 0 100 5 10
    240607030613_vu0i ATCCCAGAAAAATAGGAAGTCATTATAAATAG(+ATATAT)GATCCAATGGAACAATGTTCTTCAAATAATA NGG 10 8 17 8 24 0 100 5 10
    240607030611_cnxg AGTTATATTATTTGAAGAACATTGTTCCATTG(+ATATAT)GATCCTATTTATAATGACTTCCTATTTTTCT NGG 10 8 17 8 24 0 100 5 10
    240607030610_ouz8 AAACAGAGGATGGCTTCAAACAGGCAAGTTTG(+ATATAT)GTACCTCTTAGTGACAATTCACGGAGAAAGG NGG 10 8 17 8 24 0 100 5 10
    240607030609_g44n AATTTCCTTTCTCCGTGAATTGTCACTAAGAG(+ATATAT)GTACCAAACTTGCCTGTTTGAAGCCATCCTC NGG 10 8 17 8 24 0 100 5 10
    240607030608_tniv AAGAAGGAGGAGGAGGGATGAGTGGTCTAGTG(+ATATAT)GTACCCTCGTGCCAACAGGGTTCGTGGTGGT NGG 10 8 17 8 24 0 100 5 10
    240607030606_rcc7 TTTGCACCACCACGAACCCTGTTGGCACGAGG(+ATATAT)GTACCACTAGACCACTCATCCCTCCTCCTCC NGG 10 8 17 8 24 0 100 5 10
    240607030605_wa7m TCATAAAAAATAACTCATAGGTACAAGTAATG(+ATATAT)GTACCATATGAGTATGAGTGTAGCCTTTCTC NGG 10 8 17 8 24 0 100 5 10
    240607030604_xz8y GCTCAGAGAAAGGCTACACTCATACTCATATG(+ATATAT)GTACCATTACTTGTACCTATGAGTTATTTTT NGG 10 8 17 8 24 0 100 5 10