Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607030548_kpxv TGCTAAAGGAAATGTACTCAGCTTATAAAATG(+ATATAT)GTACCAGCTTTGCTATATTTATCGTAGCAAT NGG 10 8 17 8 24 0 100 5 10
    240607030547_lsab TGAACATTGCTACGATAAATATAGCAAAGCTG(+ATATAT)GTACCATTTTATAAGCTGAGTACATTTCCTT NGG 10 8 17 8 24 0 100 5 10
    240607030545_8dku TTGAAAAATAAAATTAATAATTTAATTGGATG(+ATATAT)GTACCAGTAACTAAACCGACTCCTGTTCATT NGG 10 8 17 8 24 0 100 5 10
    240607030544_m75y CCTTCAATGAACAGGAGTCGGTTTAGTTACTG(+ATATAT)GTACCATCCAATTAAATTATTAATTTTATTT NGG 10 8 17 8 24 0 100 5 10
    240607030543_lzkn AGTGTGAAATAAACAGGATAAAAAAGAAAATG(+ATATAT)GTACCATGAGCAGGCAGAATGCTGCAATAAT NGG 10 8 17 8 24 0 100 5 10
    240607030542_lqx5 TATCAATTATTGCAGCATTCTGCCTGCTCATG(+ATATAT)GTACCATTTTCTTTTTTATCCTGTTTATTTC NGG 10 8 17 8 24 0 100 5 10
    240607030541_6u2d GCAATCACATCGTGGCCAACCATCTGTAATGG(+ATATAT)GATCCAATGCCCTCTTCAGGTATGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240607030539_uyqo GCTGTCTTCAGACATACCTGAAGAGGGCATTG(+ATATAT)GATCCCATTACAGATGGTTGGCCACGATGTG NGG 10 8 17 8 24 0 100 5 10
    240607030537_g56o TAGCATTCAAGCCATTCTTAGGAAGGACCAGG(+ATATAT)GATCCTCTCTCATCTCTTGGGGTTGCCTTTC NGG 10 8 17 8 24 0 100 5 10
    240607030536_bwpj CCTAGGAAAGGCAACCCCAAGAGATGAGAGAG(+ATATAT)GATCCCTGGTCCTTCCTAAGAATGGCTTGAA NGG 10 8 17 8 24 0 100 5 10