Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613183857_2umz TTTTGGGGGGTGTCATATTATCACCTAGCTGG(+AGGACA)GATCCAGAGTGGGAAAGGGAAAGGCATTTTG NGG 10 8 17 8 24 0 100 5 10
    240613183854_b4sd GCTAACAATGGCCCGTAACTCCAGTTCTGGGG(+AGGACA)GATCCGACACCCTCTTCTGGCCTCTATGAGC NGG 10 8 17 8 24 0 100 5 10
    240613183853_l7i9 CATGTGCTCATAGAGGCCAGAAGAGGGTGTCG(+AGGACA)GATCCCCCAGAACTGGAGTTACGGGCCATTG NGG 10 8 17 8 24 0 100 5 10
    240613183851_zcoo GAAACAACCCAAGCTCCTCCTCCGTCCCTCTG(+AGGACA)GTACCAGATCTAACCAGGCATCCAAAGGCCT NGG 10 8 17 8 24 0 100 5 10
    240613183850_7yz9 CTCCTAGGCCTTTGGATGCCTGGTTAGATCTG(+AGGACA)GTACCAGAGGGACGGAGGAGGAGCTTGGGTT NGG 10 8 17 8 24 0 100 5 10
    240613183848_n1zh ATTGCCAGCCAATCCTTTTAGGAATAGGGCAG(+AGGACA)GTACCTTCCACTTGTATTTATTTCTGTAGCT NGG 10 8 17 8 24 0 100 5 10
    240613183847_rhjk AGAGAAGCTACAGAAATAAATACAAGTGGAAG(+AGGACA)GTACCTGCCCTATTCCTAAAAGGATTGGCTG NGG 10 8 17 8 24 0 100 5 10
    240613183845_ace5 AGGAAGGACTGCCCTTGCTATTGGAGTCAAGG(+AGGACA)GTACCCCAGTAATAGTTGTGATAATCTCCTG NGG 10 8 17 8 24 0 100 5 10
    240613183843_560r AGGATCAGGAGATTATCACAACTATTACTGGG(+AGGACA)GTACCCTTGACTCCAATAGCAAGGGCAGTCC NGG 10 8 17 8 24 0 100 5 10
    240613183842_15re GTGGGAGCTGGCTGGCGCCTCTTCTACTGCTG(+AGGACA)GTACCCCTACCAGGGACCGGGCCTTCTCCGT NGG 10 8 17 8 24 0 100 5 10