Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613183747_0y9l TCTCTCTGTGAAGGCTATGGAGAAGCCCCAGG(+AGGACA)GATCCACGCTTCCTCAAGGCGCCATAGCCAG NGG 10 8 17 8 24 0 100 5 10
    240613183746_i2bt CAGGTTGGGAGAGATAACTCAACAGTCAAGAG(+AGGACA)GATCCAGATTCAAACTCCAGAACGTACATGA NGG 10 8 17 8 24 0 100 5 10
    240613183744_7v3k AGCCATCATGTACGTTCTGGAGTTTGAATCTG(+AGGACA)GATCCTCTTGACTGTTGAGTTATCTCTCCCA NGG 10 8 17 8 24 0 100 5 10
    240613183743_naed AAATGTCCCAAGTCCCACAGTATTGGAGCTGG(+AGGACA)GTACCAGGGCCAGCCTGGTTCTTTGGTCTCT NGG 10 8 17 8 24 0 100 5 10
    240613183740_kr4f GAGAAAGAGACCAAAGAACCAGGCTGGCCCTG(+AGGACA)GTACCCAGCTCCAATACTGTGGGACTTGGGA NGG 10 8 17 8 24 0 100 5 10
    240613183738_2sla CACTGATACTGAGAAGGTGTTGGAAAACACAG(+AGGACA)GATCCATAATCTGTTGGAATAGCGGGAAAGT NGG 10 8 17 8 24 0 100 5 10
    240613183737_plho ACACAACTTTCCCGCTATTCCAACAGATTATG(+AGGACA)GATCCTGTGTTTTCCAACACCTTCTCAGTAT NGG 10 8 17 8 24 0 100 5 10
    240613183735_b3kq TCTGGGGGTGATTCCGTGTTAATCCTTGGAGG(+AGGACA)GTACCTTTCTGTTCTGATTTTTTTGTTTGTT NGG 10 8 17 8 24 0 100 5 10
    240613183733_ovjn AAACAAACAAACAAAAAAATCAGAACAGAAAG(+AGGACA)GTACCCTCCAAGGATTAACACGGAATCACCC NGG 10 8 17 8 24 0 100 5 10
    240613183731_3xsl CTTACTTTCTCCCTTTAACATACATAGGCCAG(+AGGACA)GATCCAAGCCTAATGAATAGTGTCACCTATG NGG 10 8 17 8 24 0 100 5 10