Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613183641_7ecr GACTCTTTTTCAAACTGTCCTTCCTTCTGCTG(+AGGACA)GATCCCAGTGCTGTGGAAGTCCTCAGAATCC NGG 10 8 17 8 24 0 100 5 10
    240613183639_8gbl GAAAGAGCTTTGTCTTTAAAAAAGAGATCAAG(+AGGACA)GATCCCAAAGAATACGATTCTCATATGTGAG NGG 10 8 17 8 24 0 100 5 10
    240613183637_1tyc ATTTTCTCACATATGAGAATCGTATTCTTTGG(+AGGACA)GATCCTTGATCTCTTTTTTAAAGACAAAGCT NGG 10 8 17 8 24 0 100 5 10
    240613183636_pfd6 GACCTCAACTACTCCAAAAGCTGGGCTAGGAG(+AGGACA)GATCCCAAGTTCTAGGCCAGCCAGGGCAACT NGG 10 8 17 8 24 0 100 5 10
    240613183634_ftdx CCCGAAGTTGCCCTGGCTGGCCTAGAACTTGG(+AGGACA)GATCCTCCTAGCCCAGCTTTTGGAGTAGTTG NGG 10 8 17 8 24 0 100 5 10
    240613183632_kpk0 TCCCTCACCTGGCCAGCTGCCTGCCCAACAGG(+AGGACA)GATCCTCCAGAGAGGGGGTAGCAATAGAAAC NGG 10 8 17 8 24 0 100 5 10
    240613183631_ohtk ATCCAGTTTCTATTGCTACCCCCTCTCTGGAG(+AGGACA)GATCCCTGTTGGGCAGGCAGCTGGCCAGGTG NGG 10 8 17 8 24 0 100 5 10
    240613183630_p648 TGTAACAGGATTACACAGGCTTCATTCGAGAG(+AGGACA)GATCCTACACTAAGCCGTCTCTCCACACCCA NGG 10 8 17 8 24 0 100 5 10
    240613183628_cw1m AGAGATGGGTGTGGAGAGACGGCTTAGTGTAG(+AGGACA)GATCCTCTCGAATGAAGCCTGTGTAATCCTG NGG 10 8 17 8 24 0 100 5 10
    240613183627_t539 TGAGTCTGTAATGGACACACATTCCTGGATGG(+AGGACA)GTACCCACAGCTCTACCATATCATATATTAA NGG 10 8 17 8 24 0 100 5 10