Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613183609_1amu TGGACCTTTGTCCCAGGGCAACAATTCTCCTG(+AGGACA)GTACCATGTGCACATCTAGGAAGGCCTTATT NGG 10 8 17 8 24 0 100 5 10
    240613183607_ww8t ACTCTCAAGGCATGACCTGAAATTGGAAATGG(+AGGACA)GTACCATCTCTCCCCTCAGTCACTGGAAAGC NGG 10 8 17 8 24 0 100 5 10
    240613183606_q7z6 TTGAAGCTTTCCAGTGACTGAGGGGAGAGATG(+AGGACA)GTACCCATTTCCAATTTCAGGTCATGCCTTG NGG 10 8 17 8 24 0 100 5 10
    240613183604_qa5b AAACACGGCTTTCCTAAGTAACTGTACCAATG(+AGGACA)GATCCTAAAGTCCCAAATGTAGGCCCTTGAA NGG 10 8 17 8 24 0 100 5 10
    240613183603_qf5j TTTGTTTCAAGGGCCTACATTTGGGACTTTAG(+AGGACA)GATCCATTGGTACAGTTACTTAGGAAAGCCG NGG 10 8 17 8 24 0 100 5 10
    240613183601_jmf0 GCTTTTTTCCCCTTCTAAAGTTCAAGTAAAGG(+AGGACA)GTACCTTTAAGAACAATCTGAATAGGGACTG NGG 10 8 17 8 24 0 100 5 10
    240613183600_tsxe GCCTGCAGTCCCTATTCAGATTGTTCTTAAAG(+AGGACA)GTACCCTTTACTTGAACTTTAGAAGGGGAAA NGG 10 8 17 8 24 0 100 5 10
    240613183558_pn1b ACCACATGGTGGCTCAGTACCATCTGTAATGG(+AGGACA)GATCCAATTCCCTCTTCTAGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240613183557_eb8f GCTGTCTTCAGACACACTAGAAGAGGGAATTG(+AGGACA)GATCCCATTACAGATGGTACTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240613183555_p9na TGTATGTCAGTGATGGGGCTTGCCCGGGGAAG(+AGGACA)GATCCAGAGATGAAGTAGAAGGAAAAGACTC NGG 10 8 17 8 24 0 100 5 10