Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613183554_3qpc GCAGGGAGTCTTTTCCTTCTACTTCATCTCTG(+AGGACA)GATCCTTCCCCGGGCAAGCCCCATCACTGAC NGG 10 8 17 8 24 0 100 5 10
    240613183552_knri ACGTAGGCATGCAGTCAGTAGAAGGAGGGAGG(+AGGACA)GTACCAACTGTGGTCAGGAGCTGTTCTGGGA NGG 10 8 17 8 24 0 100 5 10
    240613183551_ejzj CTACTTCCCAGAACAGCTCCTGACCACAGTTG(+AGGACA)GTACCCTCCCTCCTTCTACTGACTGCATGCC NGG 10 8 17 8 24 0 100 5 10
    240613183549_8yqb AGGGTCAGGGCTGTTGTGAGGACTAACTCCGG(+AGGACA)GTACCCTTTTCCTGTTCCTTTCCCCCTGTTC NGG 10 8 17 8 24 0 100 5 10
    240613183547_4xrx GCCTGGAACAGGGGGAAAGGAACAGGAAAAGG(+AGGACA)GTACCCGGAGTTAGTCCTCACAACAGCCCTG NGG 10 8 17 8 24 0 100 5 10
    240613183545_fk18 CCACATTTGGAAGGATGGAAGTCTAAGTGGGG(+AGGACA)GTACCTGAAGGAGCTCTGTCTCTGTCTCTGC NGG 10 8 17 8 24 0 100 5 10
    240613183544_xzit ATTGGGCAGAGACAGAGACAGAGCTCCTTCAG(+AGGACA)GTACCCCCACTTAGACTTCCATCCTTCCAAA NGG 10 8 17 8 24 0 100 5 10
    240613183542_2m3m AGTGCTTAAGAGTGCTTACTGTACTTCCAAAG(+AGGACA)GATCCAAGTCTGCTCCCATGCAGGGCTCCCA NGG 10 8 17 8 24 0 100 5 10
    240613183541_e29r CTGCATGGGAGCCCTGCATGGGAGCAGACTTG(+AGGACA)GATCCTTTGGAAGTACAGTAAGCACTCTTAA NGG 10 8 17 8 24 0 100 5 10
    240613183539_hq3g CCCTTCCCGACCGGGAGGTAATAAAATAGTTG(+AGGACA)GATCCAGTTCTGACTTGCTAGGTGCTCTAGG NGG 10 8 17 8 24 0 100 5 10