Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613183625_obk9 GTTGGTTAATATATGATATGGTAGAGCTGTGG(+AGGACA)GTACCCATCCAGGAATGTGTGTCCATTACAG NGG 10 8 17 8 24 0 100 5 10
    240613183623_2j5t CGATTTGATATCACAAGTCGAATGACTGTTGG(+AGGACA)GATCCTGGAACCAATCATTCCTGAGTTTGTG NGG 10 8 17 8 24 0 100 5 10
    240613183621_7y94 TCATTCACAAACTCAGGAATGATTGGTTCCAG(+AGGACA)GATCCCAACAGTCATTCGACTTGTGATATCA NGG 10 8 17 8 24 0 100 5 10
    240613183619_rw1g GGCTTGGATAGGTGGAATGGGAAAAGGTCCAG(+AGGACA)GATCCTAAATAGCCCCTTCAAGGGCATGGAC NGG 10 8 17 8 24 0 100 5 10
    240613183617_bicz ACCAGGTCCATGCCCTTGAAGGGGCTATTTAG(+AGGACA)GATCCTGGACCTTTTCCCATTCCACCTATCC NGG 10 8 17 8 24 0 100 5 10
    240613183616_flmr TACAATCCATTGCTTCCATGAGAGATGGGCAG(+AGGACA)GTACCTATCGGATGTGTGCTCTGCATGTTTC NGG 10 8 17 8 24 0 100 5 10
    240613183614_kqkq GGAGAGAAACATGCAGAGCACACATCCGATAG(+AGGACA)GTACCTGCCCATCTCTCATGGAAGCAATGGA NGG 10 8 17 8 24 0 100 5 10
    240613183613_t2s4 GTTTAGTAGTAATGGCAACAACATTAAGACAG(+AGGACA)GATCCTCATATGAGGACCATGTGCATGGTTG NGG 10 8 17 8 24 0 100 5 10
    240613183612_gg65 AATGTCAACCATGCACATGGTCCTCATATGAG(+AGGACA)GATCCTGTCTTAATGTTGTTGCCATTACTAC NGG 10 8 17 8 24 0 100 5 10
    240613183611_66pa TGGTAAATAAGGCCTTCCTAGATGTGCACATG(+AGGACA)GTACCAGGAGAATTGTTGCCCTGGGACAAAG NGG 10 8 17 8 24 0 100 5 10