Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613183825_v5oc TAACTGAGGCTGAACTGGTATGTGTGCTGAGG(+AGGACA)GTACCATTGATTTGATATCAACCTAGGCTAC NGG 10 8 17 8 24 0 100 5 10
    240613183824_qdyf TAGACAAGCCACTTGTTGTATCTGAGTTTTAG(+AGGACA)GTACCAGAGGGGAAATACTGCCTGATCGATT NGG 10 8 17 8 24 0 100 5 10
    240613183822_86ek ATAAAAATCGATCAGGCAGTATTTCCCCTCTG(+AGGACA)GTACCTAAAACTCAGATACAACAAGTGGCTT NGG 10 8 17 8 24 0 100 5 10
    240613183820_dkzs GCTGTAATTCCAGGCTTCTTGGAAAGCTCCGG(+AGGACA)GATCCGTTTTAGGAAAGCACCGAGAGGTAAG NGG 10 8 17 8 24 0 100 5 10
    240613183818_ufgr TCAGGCTTACCTCTCGGTGCTTTCCTAAAACG(+AGGACA)GATCCCGGAGCTTTCCAAGAAGCCTGGAATT NGG 10 8 17 8 24 0 100 5 10
    240613183817_5tgw CCCGACACCAAGGAGAGTGAAATCTGGCTCAG(+AGGACA)GATCCTTGAAGCAGCCACGTTTTCAGTGATT NGG 10 8 17 8 24 0 100 5 10
    240613183816_g2v2 GAGGAAATCACTGAAAACGTGGCTGCTTCAAG(+AGGACA)GATCCTGAGCCAGATTTCACTCTCCTTGGTG NGG 10 8 17 8 24 0 100 5 10
    240613183814_l7uc ATATTTCTAATGCATTGACTTTTCTTCTCCTG(+AGGACA)GTACCAAGTGCTAACTGTGGAATCTAGTGGA NGG 10 8 17 8 24 0 100 5 10
    240613183813_463z CAGCGTCCACTAGATTCCACAGTTAGCACTTG(+AGGACA)GTACCAGGAGAAGAAAAGTCAATGCATTAGA NGG 10 8 17 8 24 0 100 5 10
    240613183811_ua7w GTTTTGGATTTGTCCCATTTAAAGAACTAATG(+AGGACA)GATCCAGGGAAAATAAAGTATCCTTGTTTGA NGG 10 8 17 8 24 0 100 5 10