Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607025809_czyb AACCAACTTTATCTAAGGTGCTGCTGGCAGTG(+ATATAT)GATCCTAAGATCTTTTGAGCATGACCCTTAG NGG 10 8 17 8 24 0 100 5 10
    240607025807_g7zh TCTCTCTAAGGGTCATGCTCAAAAGATCTTAG(+ATATAT)GATCCACTGCCAGCAGCACCTTAGATAAAGT NGG 10 8 17 8 24 0 100 5 10
    240607025806_jldr CAATCTGCTGCTTGCTTGCGCACCCCTGCAGG(+ATATAT)GTACCCCCCTCCCCACCAAATGGGATCTCAT NGG 10 8 17 8 24 0 100 5 10
    240607025803_fp92 ACAAGATGAGATCCCATTTGGTGGGGAGGGGG(+ATATAT)GTACCCTGCAGGGGTGCGCAAGCAAGCAGCA NGG 10 8 17 8 24 0 100 5 10
    240607025802_vfxv CTTGTGGCCTCCACAGGCACTGAATGCACATG(+ATATAT)GTACCTAGACATACTCAGGCAAACACTCATA NGG 10 8 17 8 24 0 100 5 10
    240607025801_pmir ATGTGTATGAGTGTTTGCCTGAGTATGTCTAG(+ATATAT)GTACCATGTGCATTCAGTGCCTGTGGAGGCC NGG 10 8 17 8 24 0 100 5 10
    240607025800_0hj6 GCACATACGCTCACGTGTATCTTCTTTTGAAG(+ATATAT)GTACCTTCTTTACCTAGCAGTTTAACACATC NGG 10 8 17 8 24 0 100 5 10
    240607025759_uirk CTAAAGATGTGTTAAACTGCTAGGTAAAGAAG(+ATATAT)GTACCTTCAAAAGAAGATACACGTGAGCGTA NGG 10 8 17 8 24 0 100 5 10
    240607025758_zb8k AAATTGGGTGCATTCATTGACTCCAGAGTTAG(+ATATAT)GTACCCAAGCATACACTAAGAAGAACCCTCT NGG 10 8 17 8 24 0 100 5 10
    240607025756_rzhs AGTGAAGAGGGTTCTTCTTAGTGTATGCTTGG(+ATATAT)GTACCTAACTCTGGAGTCAATGAATGCACCC NGG 10 8 17 8 24 0 100 5 10