Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607025903_kpp7 ACTCCAGGGAAAGGAGCGTGTGGGGAGGGAAG(+ATATAT)GTACCAGAAGCTCATCTGATTGGCCCAACGG NGG 10 8 17 8 24 0 100 5 10
    240607025901_ispd CTAAACCGTTGGGCCAATCAGATGAGCTTCTG(+ATATAT)GTACCTTCCCTCCCCACACGCTCCTTTCCCT NGG 10 8 17 8 24 0 100 5 10
    240607025900_hnuu TTTTTAAAAACCTGTCACTACCAGAAACGGTG(+ATATAT)GTACCTCCTGCCCTTTGACTCTCATACCGTG NGG 10 8 17 8 24 0 100 5 10
    240607025858_qfix ACTTTCACGGTATGAGAGTCAAAGGGCAGGAG(+ATATAT)GTACCACCGTTTCTGGTAGTGACAGGTTTTT NGG 10 8 17 8 24 0 100 5 10
    240607025857_wyuz CTAATGCAGCCCAGTAGAGTGGAGCTCAGGAG(+ATATAT)GTACCCACAGACGGGATTTCTCTTGGTCTTG NGG 10 8 17 8 24 0 100 5 10
    240607025855_ndkw TCAGTCAAGACCAAGAGAAATCCCGTCTGTGG(+ATATAT)GTACCTCCTGAGCTCCACTCTACTGGGCTGC NGG 10 8 17 8 24 0 100 5 10
    240607025852_h1zw GCGCCAGTCCTCCGATAGACTGCGTCGCCCGG(+ATATAT)GTACCCGTGTTCCCAATAAAGCCTCTTGCTG NGG 10 8 17 8 24 0 100 5 10
    240607025851_kcvm GCAAACAGCAAGAGGCTTTATTGGGAACACGG(+ATATAT)GTACCCGGGCGACGCAGTCTATCGGAGGACT NGG 10 8 17 8 24 0 100 5 10
    240607025849_zblm TAGAAGCAGGTTCTTGGAAGTTACAATTTAAG(+ATATAT)GTACCCCCAAAAAGTTGGAAATAAAAGGAAA NGG 10 8 17 8 24 0 100 5 10
    240607025847_gpya ATTTGTTTCCTTTTATTTCCAACTTTTTGGGG(+ATATAT)GTACCTTAAATTGTAACTTCCAAGAACCTGC NGG 10 8 17 8 24 0 100 5 10