Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607025658_cj15 AGCAGGTTAAATTATCTTAACATCTTTGAATG(+ATATAT)GTACCTAAGATTTTTCTGAGCACTGTACTGG NGG 10 8 17 8 24 0 100 5 10
    240607025657_e9z1 TGATACCAGTACAGTGCTCAGAAAAATCTTAG(+ATATAT)GTACCATTCAAAGATGTTAAGATAATTTAAC NGG 10 8 17 8 24 0 100 5 10
    240607025655_x1b1 CTATGGGTTAAATGGCCCAGCTCTCTTGCCAG(+ATATAT)GATCCATTTCATTTCCTATAGCGCAGTTAGC NGG 10 8 17 8 24 0 100 5 10
    240607025654_xlma GGTGTGCTAACTGCGCTATAGGAAATGAAATG(+ATATAT)GATCCTGGCAAGAGAGCTGGGCCATTTAACC NGG 10 8 17 8 24 0 100 5 10
    240607025653_oxn1 AAATCCAGAGGGCAAAAGTGTCTGATTAAGTG(+ATATAT)GATCCTGCAAGGCAGCACCCATAAGTGAACC NGG 10 8 17 8 24 0 100 5 10
    240607025652_43i5 TCCTGGGTTCACTTATGGGTGCTGCCTTGCAG(+ATATAT)GATCCACTTAATCAGACACTTTTGCCCTCTG NGG 10 8 17 8 24 0 100 5 10
    240607025650_rntt CCCCAAGATCTTTGAGATGTCAGAGCTGTGGG(+ATATAT)GTACCTGCTCAGGAAAACCAGCCAAAGAGAA NGG 10 8 17 8 24 0 100 5 10
    240607025649_t330 ATTCTTTCTCTTTGGCTGGTTTTCCTGAGCAG(+ATATAT)GTACCCCACAGCTCTGACATCTCAAAGATCT NGG 10 8 17 8 24 0 100 5 10
    240607025648_b5o0 TCTCTTTGGAGGACTGCAGAAGATCATGTGTG(+ATATAT)GATCCAAGACATTGGAGCAAGAAGCTGTAAC NGG 10 8 17 8 24 0 100 5 10
    240607025647_xl62 TCAGTGTTACAGCTTCTTGCTCCAATGTCTTG(+ATATAT)GATCCACACATGATCTTCTGCAGTCCTCCAA NGG 10 8 17 8 24 0 100 5 10