Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607025645_ysfc CCCCAAGATCTTTGAGATGCCAGAGCTGTGGG(+ATATAT)GTACCTGCTCATGAAAACCAGCCAAAGAGAA NGG 10 8 17 8 24 0 100 5 10
    240607025644_b4vw ATTCTTTCTCTTTGGCTGGTTTTCATGAGCAG(+ATATAT)GTACCCCACAGCTCTGGCATCTCAAAGATCT NGG 10 8 17 8 24 0 100 5 10
    240607025643_2906 TCTCTTTGGAGGATAGCAGAAGATCATGTGTG(+ATATAT)GATCCAAGACATTGGAACAAGAAACTGTAAC NGG 10 8 17 8 24 0 100 5 10
    240607025641_l86r TCAGTGTTACAGTTTCTTGTTCCAATGTCTTG(+ATATAT)GATCCACACATGATCTTCTGCTATCCTCCAA NGG 10 8 17 8 24 0 100 5 10
    240607025640_yply CACAGAAGTCATCACATAGGGAAGATATGAAG(+ATATAT)GATCCTTTAGAGAAAACTTTAACAAAGTTTT NGG 10 8 17 8 24 0 100 5 10
    240607025639_aqsk CTTGTAAAACTTTGTTAAAGTTTTCTCTAAAG(+ATATAT)GATCCTTCATATCTTCCCTATGTGATGACTT NGG 10 8 17 8 24 0 100 5 10
    240607025638_wk29 AGACAGATGCCTCCACAGTTACTGCCAAACTG(+ATATAT)GTACCTAAGGACCAAAATAGCAGTCAGATGA NGG 10 8 17 8 24 0 100 5 10
    240607025636_ywpq TGTGGTCATCTGACTGCTATTTTGGTCCTTAG(+ATATAT)GTACCAGTTTGGCAGTAACTGTGGAGGCATC NGG 10 8 17 8 24 0 100 5 10
    240607025634_hq4n GATGTCATTTTAGCAGATTTTGTGTTTTGGGG(+ATATAT)GTACCACACCCTATGGCAAGCTTGGATACCT NGG 10 8 17 8 24 0 100 5 10
    240607025633_tnf3 GCACCAGGTATCCAAGCTTGCCATAGGGTGTG(+ATATAT)GTACCCCCAAAACACAAAATCTGCTAAAATG NGG 10 8 17 8 24 0 100 5 10