Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607025606_04i6 GACATTCTAGGTTTGATCTCCAACAACGGATG(+ATATAT)GTACCAATTCATTGTGTGAAGACTCCTTCAC NGG 10 8 17 8 24 0 100 5 10
    240607025605_cy9z TCCCGGTGAAGGAGTCTTCACACAATGAATTG(+ATATAT)GTACCATCCGTTGTTGGAGATCAAACCTAGA NGG 10 8 17 8 24 0 100 5 10
    240607025604_9pxz GGAGCAATGAGTCATGGTGCCTCTGGCTTCTG(+ATATAT)GTACCCCTTACAACAAGAATTCCACCCAGGC NGG 10 8 17 8 24 0 100 5 10
    240607025602_1iu8 TGCTGGCCTGGGTGGAATTCTTGTTGTAAGGG(+ATATAT)GTACCAGAAGCCAGAGGCACCATGACTCATT NGG 10 8 17 8 24 0 100 5 10
    240607025601_bds9 CTTCCTGACAAGAAGCGTGTGCTTGCAGGAAG(+ATATAT)GTACCACGCACTTTCTGACTGCAGCCCTTCC NGG 10 8 17 8 24 0 100 5 10
    240607025600_l0c6 GAAGGGGAAGGGCTGCAGTCAGAAAGTGCGTG(+ATATAT)GTACCTTCCTGCAAGCACACGCTTCTTGTCA NGG 10 8 17 8 24 0 100 5 10
    240607025558_tr7v ATGGTTCGTATACTACATCACTTGCTGATAGG(+ATATAT)GTACCTCAGAGTGCCCTAGAAACCACACGAC NGG 10 8 17 8 24 0 100 5 10
    240607025557_k7z7 AAGAAGTCGTGTGGTTTCTAGGGCACTCTGAG(+ATATAT)GTACCCTATCAGCAAGTGATGTAGTATACGA NGG 10 8 17 8 24 0 100 5 10
    240607025556_9hyb CTTGCAAGTTATGAAAATAAAGTATATAAAAG(+ATATAT)GATCCTGTGCTATTGTGTGCGTGGTTGCTGA NGG 10 8 17 8 24 0 100 5 10
    240607025555_sv72 TTGTCTCAGCAACCACGCACACAATAGCACAG(+ATATAT)GATCCTTTTATATACTTTATTTTCATAACTT NGG 10 8 17 8 24 0 100 5 10