Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607025540_fgcv TTTGGTTATTCAGATCTTATCCAACACAATTG(+ATATAT)GATCCATACTAAAAAGAAACCAAACCTTTGT NGG 10 8 17 8 24 0 100 5 10
    240607025539_acz7 TCTTCACAAAGGTTTGGTTTCTTTTTAGTATG(+ATATAT)GATCCAATTGTGTTGGATAAGATCTGAATAA NGG 10 8 17 8 24 0 100 5 10
    240607025538_5vfu TGGGAGGAACCAACCCATCTCTGTTGAGCAAG(+ATATAT)GATCCTGGATTGTATAAAAGGAGAAAGGGAA NGG 10 8 17 8 24 0 100 5 10
    240607025536_w9np CTCAGTTCCCTTTCTCCTTTTATACAATCCAG(+ATATAT)GATCCTTGCTCAACAGAGATGGGTTGGTTCC NGG 10 8 17 8 24 0 100 5 10
    240607025535_1hth ATGCAGTTCTTACATAAATAACTATTGATAGG(+ATATAT)GATCCTAAAAGTGATCTCACAGTTGTGTTTA NGG 10 8 17 8 24 0 100 5 10
    240607025534_k7zv ATGAATAAACACAACTGTGAGATCACTTTTAG(+ATATAT)GATCCCTATCAATAGTTATTTATGTAAGAAC NGG 10 8 17 8 24 0 100 5 10
    240607025532_3t7n TTTGCTGAGTCAGGATTTATATGGATACAAAG(+ATATAT)GATCCCTTCTGAAAACTGTCCATTGTGCAGG NGG 10 8 17 8 24 0 100 5 10
    240607025531_xxkh GTACGCCTGCACAATGGACAGTTTTCAGAAGG(+ATATAT)GATCCTTTGTATCCATATAAATCCTGACTCA NGG 10 8 17 8 24 0 100 5 10
    240607025530_i7k9 GAATAGTGGCTGTTCATTTATACCCTCAGCTG(+ATATAT)GTACCAACTGAGAAGCTTGCTGTCTGCTGGC NGG 10 8 17 8 24 0 100 5 10
    240607025529_qb9k AGGTAGCCAGCAGACAGCAAGCTTCTCAGTTG(+ATATAT)GTACCAGCTGAGGGTATAAATGAACAGCCAC NGG 10 8 17 8 24 0 100 5 10