Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607025458_n0dr TCCTCAGAAAATTGGACATAGTACTACCGGAG(+ATATAT)GATCCCGCAATACCTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10
    240607025457_mw7y AGTTGTTCCAGCTGGACTTCTGTATAAGCTGG(+ATATAT)GATCCTGAAGAAGTAGGTTCCAACAGATGTG NGG 10 8 17 8 24 0 100 5 10
    240607025455_av7h GCCAGCACATCTGTTGGAACCTACTTCTTCAG(+ATATAT)GATCCCAGCTTATACAGAAGTCCAGCTGGAA NGG 10 8 17 8 24 0 100 5 10
    240607025454_9vva AGGCCTCCCCAAGTATTGCGGGGCATTCTGGG(+ATATAT)GATCCAGTTTAGGTTCAGTAATAAAGACACA NGG 10 8 17 8 24 0 100 5 10
    240607025453_0xto GTCTCTGTGTCTTTATTACTGAACCTAAACTG(+ATATAT)GATCCCCAGAATGCCCCGCAATACTTGGGGA NGG 10 8 17 8 24 0 100 5 10
    240607025451_34jo TTCTGGATATATGCCCAGGAGAGGTATTGCGG(+ATATAT)GATCCTCCGGTAGTACTATGTCCAATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240607025449_g4u0 TCCTCAGAAAATTGGACATAGTACTACCGGAG(+ATATAT)GATCCCGCAATACCTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10
    240607025448_f2fr GTTAAAGGACTCCTGAGAAGTTACAGACTCAG(+ATATAT)GATCCTTATAATTATTTGGGTTTTAGACTTA NGG 10 8 17 8 24 0 100 5 10
    240607025447_kjy4 ATCTGTAAGTCTAAAACCCAAATAATTATAAG(+ATATAT)GATCCTGAGTCTGTAACTTCTCAGGAGTCCT NGG 10 8 17 8 24 0 100 5 10
    240607025446_xoh1 ATCCCTTCTACTAAGTGCATACATGTAGTTTG(+ATATAT)GATCCATATTTCAATTCACTGTCAAGTTTCT NGG 10 8 17 8 24 0 100 5 10