Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607025405_sb7s AATAGTTAGCAACCAGGTGGTTTGGGGAATGG(+ATATAT)GATCCCTGTCTATTGAAACAATATCCAGTTT NGG 10 8 17 8 24 0 100 5 10
    240607025403_5gc7 ACTCCCCAGAACATGCCACATCCCTTAATGGG(+ATATAT)GATCCCAAATGAGACCTGAGGTGAAAGACAT NGG 10 8 17 8 24 0 100 5 10
    240607025402_9xgt CTTCAATGTCTTTCACCTCAGGTCTCATTTGG(+ATATAT)GATCCCCATTAAGGGATGTGGCATGTTCTGG NGG 10 8 17 8 24 0 100 5 10
    240607025400_ax4i ACTTCCCCAATTCAGTATCCCCTGACAAGTGG(+ATATAT)GATCCTGCACTCAAGTCCCTTAAATATATAC NGG 10 8 17 8 24 0 100 5 10
    240607025359_xrev TCATAGTATATATTTAAGGGACTTGAGTGCAG(+ATATAT)GATCCCACTTGTCAGGGGATACTGAATTGGG NGG 10 8 17 8 24 0 100 5 10
    240607025357_z61e GCCTCCCCAACTCAGTAGCCCCTGACAAGTGG(+ATATAT)GATCCTGCACTCAAGTCCCTTAAATCCATAC NGG 10 8 17 8 24 0 100 5 10
    240607025356_vhs4 TCATAGTATGGATTTAAGGGACTTGAGTGCAG(+ATATAT)GATCCCACTTGTCAGGGGCTACTGAGTTGGG NGG 10 8 17 8 24 0 100 5 10
    240607025355_andn GATCCCTCTGCACCACGTGTCTTAGAATTAAG(+ATATAT)GATCCAAAAGAAGAAAAAAAGTTACCATGGT NGG 10 8 17 8 24 0 100 5 10
    240607025354_0ql3 TCAACACCATGGTAACTTTTTTTCTTCTTTTG(+ATATAT)GATCCTTAATTCTAAGACACGTGGTGCAGAG NGG 10 8 17 8 24 0 100 5 10
    240607025352_m36z TCAACCTTCTGAGGCATGTGCTACCATGCCTG(+ATATAT)GATCCCAAGTTTTTCCTTAAGTGGTCTTGAT NGG 10 8 17 8 24 0 100 5 10