Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607025336_5cag GATGTCTTCAGACACACCAGAAGAGGGCATCG(+ATATAT)GATCCCATTACAGATGGTTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240607025335_pom2 CTCAGGGAGAGACTTGCAAAACAAACTGTGTG(+ATATAT)GATCCTGCAGACCAACATTAAAGGTTGACCT NGG 10 8 17 8 24 0 100 5 10
    240607025334_ok9o GCCAAAGGTCAACCTTTAATGTTGGTCTGCAG(+ATATAT)GATCCACACAGTTTGTTTTGCAAGTCTCTCC NGG 10 8 17 8 24 0 100 5 10
    240607025332_5bsl GGCCCTCAGGCCCACACTCCCTAGGTGGCCTG(+ATATAT)GATCCTGCAGACCTTGCCCATTCTCCAGTGT NGG 10 8 17 8 24 0 100 5 10
    240607025331_kqd7 AGGAGACACTGGAGAATGGGCAAGGTCTGCAG(+ATATAT)GATCCAGGCCACCTAGGGAGTGTGGGCCTGA NGG 10 8 17 8 24 0 100 5 10
    240607025329_0m7s GAGTTCATCTGCCCTTCATGTTTTATGCGAAG(+ATATAT)GTACCTCCTTTGTACTTAGAAAAAAAAAGAC NGG 10 8 17 8 24 0 100 5 10
    240607025328_r9z9 TAAATGTCTTTTTTTTTCTAAGTACAAAGGAG(+ATATAT)GTACCTTCGCATAAAACATGAAGGGCAGATG NGG 10 8 17 8 24 0 100 5 10
    240607025326_yphu ATGACTGATATCTATAGCCCAGGAACCGCCTG(+ATATAT)GATCCAGGATTGTGAAACTAAGCCCGTGGGA NGG 10 8 17 8 24 0 100 5 10
    240607025325_8j4d GTCCCTCCCACGGGCTTAGTTTCACAATCCTG(+ATATAT)GATCCAGGCGGTTCCTGGGCTATAGATATCA NGG 10 8 17 8 24 0 100 5 10
    240607025324_lvnt TCCTCTACAAGGATACTGTGGCTAATGCACAG(+ATATAT)GTACCCTGATTGCAGAGAAAACTCTGGGCAT NGG 10 8 17 8 24 0 100 5 10