Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607025251_zm3a AAGCTATGAAGGGAGATTGGTACCCACACCAG(+ATATAT)GATCCAGAAGCCCCCAACCACGTGCTGAGGC NGG 10 8 17 8 24 0 100 5 10
    240607025250_7mgb TGGATGAAGCATCTTTAACCTCATAATAAATG(+ATATAT)GATCCAGATTTTACCTCAAAGAATTATATTC NGG 10 8 17 8 24 0 100 5 10
    240607025248_avju AATAAGAATATAATTCTTTGAGGTAAAATCTG(+ATATAT)GATCCATTTATTATGAGGTTAAAGATGCTTC NGG 10 8 17 8 24 0 100 5 10
    240607025247_uv94 ATCATAGAACAACTTGCACTTTCTGCTATATG(+ATATAT)GATCCAAGATATTGAACTGAGTGATCAGGTT NGG 10 8 17 8 24 0 100 5 10
    240607025246_6h6a TTTCAAACCTGATCACTCAGTTCAATATCTTG(+ATATAT)GATCCATATAGCAGAAAGTGCAAGTTGTTCT NGG 10 8 17 8 24 0 100 5 10
    240607025245_8p2b TTTTCATCCTCCTGGGGATGTGGAAGCTGCAG(+ATATAT)GATCCCTGAGATGAAGTGCAGTGTGGAGCCT NGG 10 8 17 8 24 0 100 5 10
    240607025244_d1n0 GACACAGGCTCCACACTGCACTTCATCTCAGG(+ATATAT)GATCCTGCAGCTTCCACATCCCCAGGAGGAT NGG 10 8 17 8 24 0 100 5 10
    240607025242_oxjm GCCCATGCCACTGTGGCCCTTAATATGCACAG(+ATATAT)GTACCCAAGGGTGGCACAAAAGCATGTACCT NGG 10 8 17 8 24 0 100 5 10
    240607025240_t6xh ACTTGAGGTACATGCTTTTGTGCCACCCTTGG(+ATATAT)GTACCTGTGCATATTAAGGGCCACAGTGGCA NGG 10 8 17 8 24 0 100 5 10
    240607025239_uyuq GAAAAAGAAATGAAGATTTCCCTCCTCAAATG(+ATATAT)GTACCCTGGGGACAGCTTGTCTAGTTATGTG NGG 10 8 17 8 24 0 100 5 10