Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607025208_eoqg CCCACTAAAAATCCACTCACGGGAACCACTGG(+ATATAT)GATCCACAGGTCCAACTGGTTTCTCATCTGC NGG 10 8 17 8 24 0 100 5 10
    240607025206_skbt AGCGTCCTTTAGAAAATGAAAGCATTGCCCGG(+ATATAT)GATCCGTTTTTTGAAAGAGCAGTGCAGTCGG NGG 10 8 17 8 24 0 100 5 10
    240607025205_pzxa CTTAGCCGACTGCACTGCTCTTTCAAAAAACG(+ATATAT)GATCCCGGGCAATGCTTTCATTTTCTAAAGG NGG 10 8 17 8 24 0 100 5 10
    240607025203_8ntd AGCACTGTAATAGTGGGTCCTAGGCAGCCACG(+ATATAT)GTACCCAGGACCACGTTCTCCAGCGTTTTCC NGG 10 8 17 8 24 0 100 5 10
    240607025200_fvfe GATCTGGAAAACGCTGGAGAACGTGGTCCTGG(+ATATAT)GTACCGTGGCTGCCTAGGACCCACTATTACA NGG 10 8 17 8 24 0 100 5 10
    240607025159_ci37 CTTGCCTTGTGGCCACATACATAAGCATTGAG(+ATATAT)GTACCCCAAGAGTCACCCTGTCCTCTGTCCC NGG 10 8 17 8 24 0 100 5 10
    240607025157_hos5 CTTGGGGGACAGAGGACAGGGTGACTCTTGGG(+ATATAT)GTACCTCAATGCTTATGTATGTGGCCACAAG NGG 10 8 17 8 24 0 100 5 10
    240607025156_28mj TACGCCAGGCATGAGGGTCTGGCGTGTGCCTG(+ATATAT)GATCCTGTGCTCCTCCTTATAGGCACCCCAC NGG 10 8 17 8 24 0 100 5 10
    240607025154_nhfx GAGTGGTGGGGTGCCTATAAGGAGGAGCACAG(+ATATAT)GATCCAGGCACACGCCAGACCCTCATGCCTG NGG 10 8 17 8 24 0 100 5 10
    240607025153_ahm8 GAAAGGTGTGCACACTGGCATCACGGTCGACG(+ATATAT)GATCCACTGGGCATACATCAAGTGAGCGCAA NGG 10 8 17 8 24 0 100 5 10