Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607025152_p6nm CCCAATTGCGCTCACTTGATGTATGCCCAGTG(+ATATAT)GATCCGTCGACCGTGATGCCAGTGTGCACAC NGG 10 8 17 8 24 0 100 5 10
    240607025150_arhu GAGTTCTGTTAACTCGTGGCTGATGTGTCCTG(+ATATAT)GATCCTAGGCACCGGTGGAGAAAGGTGTAAG NGG 10 8 17 8 24 0 100 5 10
    240607025148_oeeq TATTTCTTACACCTTTCTCCACCGGTGCCTAG(+ATATAT)GATCCAGGACACATCAGCCACGAGTTAACAG NGG 10 8 17 8 24 0 100 5 10
    240607025146_etf4 AGAGCCACCTCGTGCATGGAGAGTGAGTCTGG(+ATATAT)GATCCAGTGAGAGTCACAATGGCAAGGACAT NGG 10 8 17 8 24 0 100 5 10
    240607025145_3n2o TATTGATGTCCTTGCCATTGTGACTCTCACTG(+ATATAT)GATCCCAGACTCACTCTCCATGCACGAGGTG NGG 10 8 17 8 24 0 100 5 10
    240607025144_ohsw TCAAGTTTATTAGACGAAAGCATAACCAAAAG(+ATATAT)GTACCTGAAGGGAACTGAGTTTTTCACAGTC NGG 10 8 17 8 24 0 100 5 10
    240607025143_utwx TGATGGACTGTGAAAAACTCAGTTCCCTTCAG(+ATATAT)GTACCTTTTGGTTATGCTTTCGTCTAATAAA NGG 10 8 17 8 24 0 100 5 10
    240607025142_okpd TATTTTGTGGCCACTTCATTTGACAACTTGAG(+ATATAT)GTACCACTGCTCTATGTCCATTGGATCTGGG NGG 10 8 17 8 24 0 100 5 10
    240607025140_yps4 TAGGCCCCAGATCCAATGGACATAGAGCAGTG(+ATATAT)GTACCTCAAGTTGTCAAATGAAGTGGCCACA NGG 10 8 17 8 24 0 100 5 10
    240607025139_wfv4 TCCTGCTTCCAGTTGATTGTTGGGGTTGGTTG(+ATATAT)GTACCAAAGGTGGGGTGTCTGAAAGGGAGTA NGG 10 8 17 8 24 0 100 5 10