Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607025053_yokk ATTTGAAATACTTTCCACCCTTGTCCCTCCAG(+ATATAT)GATCCTGGTGATGCAAATTTTCTAGGACCAT NGG 10 8 17 8 24 0 100 5 10
    240607025052_2bhs TCAGTGTACTGGAGGAGAAAATCAAACTGTTG(+ATATAT)GATCCCAAGTGGCTACATCACCATGAAGAGA NGG 10 8 17 8 24 0 100 5 10
    240607025051_v4cz CTGATTCTCTTCATGGTGATGTAGCCACTTGG(+ATATAT)GATCCAACAGTTTGATTTTCTCCTCCAGTAC NGG 10 8 17 8 24 0 100 5 10
    240607025049_uoxp TTGAGTCCACTCAGTGCCTCCCTGGAGACCTG(+ATATAT)GATCCATCCTGCCCTGGCCATTACCTTGTTG NGG 10 8 17 8 24 0 100 5 10
    240607025048_ud0w CATGTCAACAAGGTAATGGCCAGGGCAGGATG(+ATATAT)GATCCAGGTCTCCAGGGAGGCACTGAGTGGA NGG 10 8 17 8 24 0 100 5 10
    240607025046_zmha TAATGCCTAAGCGCTCCTTGGCTCCCAGAGTG(+ATATAT)GATCCTGCCTAGTTTGTCACTGTCACTTTAG NGG 10 8 17 8 24 0 100 5 10
    240607025045_ifm8 TCTTTCTAAAGTGACAGTGACAAACTAGGCAG(+ATATAT)GATCCACTCTGGGAGCCAAGGAGCGCTTAGG NGG 10 8 17 8 24 0 100 5 10
    240607025043_1k5m TCCTCAGAAAATTGGACATAGTACTACCGGAG(+ATATAT)GATCCCGCAATACCTCTCCTGGGAATATATC NGG 10 8 17 8 24 0 100 5 10
    240607025042_x0my TTCTGGATATATTCCCAGGAGAGGTATTGCGG(+ATATAT)GATCCTCCGGTAGTACTATGTCCAATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240607025040_thz3 GGATGGACCATCTAGTGATTGCCATATGCAGG(+ATATAT)GATCCATCCCATAATCAGCTTCCAAATGCTG NGG 10 8 17 8 24 0 100 5 10