Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607025108_l2ml GCAAATGGTATCTCGTATTAGTAGCTCTCCTG(+ATATAT)GATCCCTAAAATTCTTTCCGATAGGATTGCC NGG 10 8 17 8 24 0 100 5 10
    240607025107_gzpv TAGCAACAAGAATCCAAGTGTACACTGTGGTG(+ATATAT)GATCCAGCTTCAAAGCAAACTGTTGCCTCCA NGG 10 8 17 8 24 0 100 5 10
    240607025105_qz7q GTGTTTGGAGGCAACAGTTTGCTTTGAAGCTG(+ATATAT)GATCCACCACAGTGTACACTTGGATTCTTGT NGG 10 8 17 8 24 0 100 5 10
    240607025104_iyku GTGAAATCAAGATTAGTTAAACCATCTAAACG(+ATATAT)GATCCATAACCCCTAAGGAAATACAAAGAGT NGG 10 8 17 8 24 0 100 5 10
    240607025103_m3ys TAATGACTCTTTGTATTTCCTTAGGGGTTATG(+ATATAT)GATCCGTTTAGATGGTTTAACTAATCTTGAT NGG 10 8 17 8 24 0 100 5 10
    240607025101_y0ii GGAAAGACCATCCAGAGACTGTCCTACCAGGG(+ATATAT)GATCCATCCCATATACAACCACCAAACACAG NGG 10 8 17 8 24 0 100 5 10
    240607025059_g70b AGTGTCTGTGTTTGGTGGTTGTATATGGGATG(+ATATAT)GATCCCCTGGTAGGACAGTCTCTGGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240607025058_fn4c CAAAGGCAGCTGCAGAGCTCAGCCTTGGGTAG(+ATATAT)GATCCTGTCTGAGGACACTGACTGAGGACAT NGG 10 8 17 8 24 0 100 5 10
    240607025057_kxdb CAGGGATGTCCTCAGTCAGTGTCCTCAGACAG(+ATATAT)GATCCTACCCAAGGCTGAGCTCTGCAGCTGC NGG 10 8 17 8 24 0 100 5 10
    240607025055_7vqg CTGCCATGGTCCTAGAAAATTTGCATCACCAG(+ATATAT)GATCCTGGAGGGACAAGGGTGGAAAGTATTT NGG 10 8 17 8 24 0 100 5 10