Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607025012_7bx0 CTTTTAAAATAAACCAGTGATGGTTTTATAAG(+ATATAT)GTACCTTTTCCTGAGTTCTAGGAACCATTCT NGG 10 8 17 8 24 0 100 5 10
    240607025010_0ti1 GACTTGTGAAAATCAGCTCTCCTCACCATGTG(+ATATAT)GATCCTGAAGATTGAATTTACGTCTTTCAGG NGG 10 8 17 8 24 0 100 5 10
    240607025009_w9g6 CCAAGCCTGAAAGACGTAAATTCAATCTTCAG(+ATATAT)GATCCACATGGTGAGGAGAGCTGATTTTCAC NGG 10 8 17 8 24 0 100 5 10
    240607025008_ccx7 TAGCCAATGACACGTTTTTGACTCAGCGAATG(+ATATAT)GTACCAGTCACAGGTCCCATCTTGTAGAGTG NGG 10 8 17 8 24 0 100 5 10
    240607025007_j1s9 AAGCCCACTCTACAAGATGGGACCTGTGACTG(+ATATAT)GTACCATTCGCTGAGTCAAAAACGTGTCATT NGG 10 8 17 8 24 0 100 5 10
    240607025006_4e5q GTCAGAGAGGGCCATCTCATCAGCTGGGAAAG(+ATATAT)GTACCTGCCAGCAAGGCTGATAATGTGCTTG NGG 10 8 17 8 24 0 100 5 10
    240607025004_jyi2 GGGATCAAGCACATTATCAGCCTTGCTGGCAG(+ATATAT)GTACCTTTCCCAGCTGATGAGATGGCCCTCT NGG 10 8 17 8 24 0 100 5 10
    240607025002_fsf1 AAACATCTCCAAACCCATAAAGAGATCGCCTG(+ATATAT)GTACCGAAACCAGATGAGAAAAGGGGAGGAG NGG 10 8 17 8 24 0 100 5 10
    240607025001_jn6x GCTGCCTCCTCCCCTTTTCTCATCTGGTTTCG(+ATATAT)GTACCAGGCGATCTCTTTATGGGTTTGGAGA NGG 10 8 17 8 24 0 100 5 10
    240607024958_pwlh CCAGCAAGGCTGATAATGTGCTTGATCCCCAG(+ATATAT)GATCCACAGCGTGGAAGGAAAGAACCCATGT NGG 10 8 17 8 24 0 100 5 10