Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607024943_mtbl CGGGGTGTGACGGCGGGGACGGTGGTGGACAG(+ATATAT)GTACCAAAGCTATTTTCTCATCTGTCCTGTG NGG 10 8 17 8 24 0 100 5 10
    240607024941_dnvn AAAAATCCCCATACAAAAGTGAAGTCCCATTG(+ATATAT)GTACCCCTGCCATCATCTCCTAGTGGCTATT NGG 10 8 17 8 24 0 100 5 10
    240607024939_mgve CACAGAATAGCCACTAGGAGATGATGGCAGGG(+ATATAT)GTACCAATGGGACTTCACTTTTGTATGGGGA NGG 10 8 17 8 24 0 100 5 10
    240607024938_g6v2 TATAATCCTGAACATGAAGTGTTTCTAAAAGG(+ATATAT)GATCCTCCTTCCCAGTAGACAGAAAAAAGTC NGG 10 8 17 8 24 0 100 5 10
    240607024936_j7ep GGCTCGACTTTTTTCTGTCTACTGGGAAGGAG(+ATATAT)GATCCCTTTTAGAAACACTTCATGTTCAGGA NGG 10 8 17 8 24 0 100 5 10
    240607024935_tkz4 TTTAGTCTTCTGCAACAACAGCCACCAAAGTG(+ATATAT)GATCCTAGGCGTTCCTGGCAGTCACCACTGG NGG 10 8 17 8 24 0 100 5 10
    240607024934_2p5b GCTTGCCAGTGGTGACTGCCAGGAACGCCTAG(+ATATAT)GATCCACTTTGGTGGCTGTTGTTGCAGAAGA NGG 10 8 17 8 24 0 100 5 10
    240607024931_kkc4 TGGACTTTCATAGGACATAAGCTTGAACCCCG(+ATATAT)GTACCGCTCAGCAGAACAGTTGGGTTCTCAG NGG 10 8 17 8 24 0 100 5 10
    240607024930_y2s4 AGGCACTGAGAACCCAACTGTTCTGCTGAGCG(+ATATAT)GTACCGGGGTTCAAGCTTATGTCCTATGAAA NGG 10 8 17 8 24 0 100 5 10
    240607024928_vnf2 AGAACCTGCCACAGCTTAACACACCTTATCTG(+ATATAT)GTACCTGTCTGAAAGCACTCCCTGGGCCTAT NGG 10 8 17 8 24 0 100 5 10