Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607025046_zmha TAATGCCTAAGCGCTCCTTGGCTCCCAGAGTG(+ATATAT)GATCCTGCCTAGTTTGTCACTGTCACTTTAG NGG 10 8 17 8 24 0 100 5 10
    240607025045_ifm8 TCTTTCTAAAGTGACAGTGACAAACTAGGCAG(+ATATAT)GATCCACTCTGGGAGCCAAGGAGCGCTTAGG NGG 10 8 17 8 24 0 100 5 10
    240607025043_1k5m TCCTCAGAAAATTGGACATAGTACTACCGGAG(+ATATAT)GATCCCGCAATACCTCTCCTGGGAATATATC NGG 10 8 17 8 24 0 100 5 10
    240607025042_x0my TTCTGGATATATTCCCAGGAGAGGTATTGCGG(+ATATAT)GATCCTCCGGTAGTACTATGTCCAATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240607025040_thz3 GGATGGACCATCTAGTGATTGCCATATGCAGG(+ATATAT)GATCCATCCCATAATCAGCTTCCAAATGCTG NGG 10 8 17 8 24 0 100 5 10
    240607025039_g2a5 GGTGTCAGCATTTGGAAGCTGATTATGGGATG(+ATATAT)GATCCCTGCATATGGCAATCACTAGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240607025037_xprm ACAGAGTTTGAGAGCACAGAGGTGATGGGGAG(+ATATAT)GATCCAAGGTTGGAGATGATGATAGCCTTCT NGG 10 8 17 8 24 0 100 5 10
    240607025036_nvdt GCACTAGAAGGCTATCATCATCTCCAACCTTG(+ATATAT)GATCCTCCCCATCACCTCTGTGCTCTCAAAC NGG 10 8 17 8 24 0 100 5 10
    240607025034_tszm TATAGTGCAGAATGGCTGACATGCCCATATAG(+ATATAT)GTACCAGGGGGAGGTTTTTTCACCCTTGTCA NGG 10 8 17 8 24 0 100 5 10
    240607025032_4bco TGCTTTGACAAGGGTGAAAAAACCTCCCCCTG(+ATATAT)GTACCTATATGGGCATGTCAGCCATTCTGCA NGG 10 8 17 8 24 0 100 5 10