Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607024857_838x ACGTTCTGTGGGGGGATTTGTCCTCCCTTGAG(+ATATAT)GATCCCTGTTTTCTGTAGGTGATCCTGTATG NGG 10 8 17 8 24 0 100 5 10
    240607024856_abdt CGGGCCCTGTCCTGCTCTCGCATTGCTTGATG(+ATATAT)GATCCTACACCAGTCTAAAAACTTACATAAG NGG 10 8 17 8 24 0 100 5 10
    240607024855_1jx3 GTCTGCTTATGTAAGTTTTTAGACTGGTGTAG(+ATATAT)GATCCATCAAGCAATGCGAGAGCAGGACAGG NGG 10 8 17 8 24 0 100 5 10
    240607024854_6krl TAAGGCAGCAGCCCCAGGCAGCATTTAAGCGG(+ATATAT)GATCCTTAGCAAGACAGAATCCAGCAAAACC NGG 10 8 17 8 24 0 100 5 10
    240607024852_meib GAAAGGGTTTTGCTGGATTCTGTCTTGCTAAG(+ATATAT)GATCCCGCTTAAATGCTGCCTGGGGCTGCTG NGG 10 8 17 8 24 0 100 5 10
    240607024851_b1b0 GATCTCGTTTACCTCCTGTTTAGTGGGCTTTG(+ATATAT)GATCCTCCCTGTTAGTTCTTCATGTGGTTTT NGG 10 8 17 8 24 0 100 5 10
    240607024850_ugj9 TAAGTAAAACCACATGAAGAACTAACAGGGAG(+ATATAT)GATCCAAAGCCCACTAAACAGGAGGTAAACG NGG 10 8 17 8 24 0 100 5 10
    240607024848_hsse GCTGCATCTCAGATTGTGGGGGGTGGGAGGGG(+ATATAT)GATCCTAACCTCAGGCACAATAGCATTCATA NGG 10 8 17 8 24 0 100 5 10
    240607024847_c9xl CTGTTTATGAATGCTATTGTGCCTGAGGTTAG(+ATATAT)GATCCCCCTCCCACCCCCCACAATCTGAGAT NGG 10 8 17 8 24 0 100 5 10
    240607024846_hp3d ATGTGTGCCCCAAATACTCATGCTGTTCAAAG(+ATATAT)GATCCTCTTGTCTGCTTCGCATAGGGTGGAG NGG 10 8 17 8 24 0 100 5 10